In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection

被引:37
作者
Aung, Hnin-Pwint [1 ,2 ]
Pyell, Ute [3 ]
机构
[1] Univ Tunku Abdul Rahman, Dept Chem Sci, Fac Sci, Kampar 31900, Malaysia
[2] Univ Tunku Abdul Rahman, Ctr Biodivers Res, Kampar 31900, Malaysia
[3] Univ Marburg, Dept Chem, Hans Meerwein Str, D-35032 Marburg, Germany
关键词
In-capillary derivatization; Capillary electrophoresis; Monosodium glutamate; Sorbic acid; Benzoic acid; o-Phthalaldehyde; SEPARATION; STACKING; SUPPLEMENTS; PRINCIPLES; FOODSTUFFS; STABILITY; INJECTION; TOOL; CE;
D O I
10.1016/j.chroma.2016.04.033
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
For the rapid simultaneous determination of monosodium glutamate (MSG), benzoic acid (BA), and sorbic acid (SA) in canned food and other processed food samples, we developed a method that combines in capillary derivatization with separation by capillary electrophoresis. This method employs the rapid derivatization of MSG with o-phthalaldehyde (OPA) in the presence of 3-mercaptopropionic acid (3-MPA) and enables the detection of the resulting OPA-MSG derivative and of non-derivatized BA and SA at 230 nm. The composition of the background electrolyte and the parameters of derivatization and separation are as follows: 25 mM borax containing 5 mM OPA and 6 mM 3-MPA, separation voltage 25 mV, injection at 30 mbar for 20 s, and column temperature 25 degrees C. Because of the high reaction rate and suitably adapted effective electrophoretic mobilities, band broadening due to the derivatization reaction at the start of the separation process is kept to a minimum. The optimized method is validated with respect to LOD, LOQ linearity, recovery, and precision. This method can be applied to real samples such as soy, fish, oyster and sweet and sour chili sauces after application of appropriate clean-up steps. Mechanisms of zone broadening and zone focusing are discussed showing the validity of the employed theoretical approach regarding the dependence of the peak shape for OPA-MSG on the concentration of MSG in the sample. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:156 / 165
页数:10
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