Factor V Leiden and FII 20210 testing in thromboembolic disorders

被引：5
|
作者：
Pajic, Tadej [1 ]
机构：
[1] Univ Med Ctr Ljubljana, Dept Haematol, Div Internal Med, Ljubljana, Slovenia
来源：
CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2010年 / 48卷
关键词：
Factor V Leiden; FII; 20210; quantitative real-time PCR; thromboembolic disorders; ACTIVATED PROTEIN-C; REAL-TIME PCR; POOR ANTICOAGULANT RESPONSE; VENOUS THROMBOEMBOLISM; PROTHROMBIN GENE; APC-RESISTANCE; INHERITED RESISTANCE; MULTIPLEX PCR; 3-UNTRANSLATED REGION; MOLECULAR-MECHANISMS;
D O I：
10.1515/CCLM.2010.372
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Factor V Leiden and prothrombin (F2) c.20210G>A mutation detection are very important in order to define the increased relative risk for venous thromboembolism in selected patients. Use of DNA-based methods to detect both mutations has become widely available in clinical diagnostic laboratories, including fluorescence-based quantitative real-time PCR (qPCR). The latter is a rapid, simple, robust and reliable method to identify genotypes of interest. There are several chemistries used for qPCR; this article describes their principles and applicability for Factor V Leiden and prothrombin (F2) c.20210G>A mutation detection. Clin Chem Lab Med 2010;48:S79-87.
引用
收藏
页码：S79 / S87
页数：9
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