Evaluation of the growth and osteogenic differentiation of ASCs cultured with PL and seeded on PLGA scaffolds

被引：5

作者：

Awidi, Abdalla ^{[1
]}

Ababneh, Nidaa ^{[1
]}

Alkilani, Hussein ^{[2
]}

Salah, Bariqa ^{[3
]}

Nazzal, Shymaa ^{[1
]}

Zoghool, Maisaa ^{[1
]}

Shomaf, Maha ^{[4
]}

机构：

[1] Univ Jordan, Cell Therapy Ctr, Amman 11942, Jordan

[2] Univ Jordan, Dept Chem, Amman 11942, Jordan

[3] Jordan Univ Hosp, Dept Plast Surg, Amman, Jordan

[4] Univ Jordan, Dept Pathol Microbiol & Forens Med, Amman 11942, Jordan

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2015年 / 26卷 / 02期

关键词：

HUMAN PLATELET LYSATE; FETAL BOVINE SERUM; BONE-FORMATION; STEM-CELLS; STROMAL CELLS; EXPANSION; HYDROGELS; MARROW;

D O I：

10.1007/s10856-015-5404-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Scaffold serves as an important component of tissue engineering, which facilitates cell attachment, proliferation and differentiation of cultured cells. In this study we aimed to use platelet lysates as a substitute for FBS in culturing and proliferation of human adipose tissue-derived stromal cells (ASCs), which constitute a promising source for cell therapy. We characterized ASCs in the presence of PL, and then we seeded them onto poly(lactic-co-glycolic acid) (PLGA) scaffolds, osteogenic media was used to induce their proliferation and osteogenic differentiation. Gene expression analysis revealed higher expression of osteogenic related genes, immunohistochemical staining showed proper cell attachment, growth and collagen matrix formation with the ability to induce vascularization. In conclusion, expansion of ASCs in PL-supplemented medium could promote cell proliferation and osteogenic differentiation of cells seeded on PLGA scaffolds, therefore it could be considered as a suitable and effective substitute for FBS to be used in clinical applications.

引用

页数：9

共 25 条

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