The structure of Jann_2411 (DUF1470) from Jannaschia sp. at 1.45 Å resolution reveals a new fold (the ABATE domain) and suggests its possible role as a transcription regulator

被引：10

作者：

Bakolitsa, Constantina ^{[1
]}

Bateman, Alex ^{[2
]}

Jin, Kevin K. ^{[3
]}

McMullan, Daniel ^{[4
]}

Krishna, S. Sri ^{[1
,5
]}

Miller, Mitchell D. ^{[3
]}

Abdubek, Polat ^{[4
]}

Acosta, Claire ^{[4
]}

Astakhova, Tamara ^{[5
]}

Axelrod, Herbert L. ^{[3
]}

Burra, Prasad ^{[1
]}

Carlton, Dennis ^{[6
]}

Chiu, Hsiu-Ju ^{[3
]}

Clayton, Thomas ^{[6
]}

Das, Debanu ^{[3
]}

Deller, Marc C. ^{[6
]}

Duan, Lian ^{[5
]}

Elias, Ylva ^{[6
]}

Feuerhelm, Julie ^{[4
]}

Grant, Joanna C. ^{[4
]}

Grzechnik, Anna ^{[6
]}

Grzechnik, Slawomir K. ^{[5
]}

Han, Gye Won ^{[6
]}

Jaroszewski, Lukasz ^{[1
,5
]}

Klock, Heath E. ^{[4
]}

Knuth, Mark W. ^{[4
]}

Kozbial, Piotr ^{[1
]}

Kumar, Abhinav ^{[3
]}

Marciano, David ^{[6
]}

Morse, Andrew T. ^{[5
]}

Murphy, Kevin D. ^{[6
]}

Nigoghossian, Edward ^{[4
]}

Okach, Linda ^{[4
]}

Oommachen, Silvya ^{[3
]}

Paulsen, Jessica ^{[4
]}

Reyes, Ron ^{[3
]}

Rife, Christopher L. ^{[3
]}

Sefcovic, Natasha ^{[1
]}

Tien, Henry ^{[5
]}

Trame, Christine B. ^{[3
]}

Trout, Christina V. ^{[6
]}

van den Bedem, Henry ^{[3
]}

Weekes, Dana ^{[1
]}

White, Aprilfawn ^{[4
]}

Xu, Qingping ^{[3
]}

Hodgson, Keith O.

Wooley, John ^{[5
]}

Elsliger, Marc-Andre ^{[6
]}

Deacon, Ashley M. ^{[3
]}

Godzik, Adam ^{[1
,5
]}

机构：

[1] Burnham Inst Med Res, Program Bioinformat & Syst Biol, La Jolla, CA USA

[2] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England

[3] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA USA

[4] Novartis Res Fdn, Prot Sci Dept, Genom Inst, San Diego, CA USA

[5] Univ Calif San Diego, Ctr Res Biol Syst, La Jolla, CA 92093 USA

[6] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

基金：

美国国家卫生研究院; 英国惠康基金;

关键词：

THERMOTOGA-MARITIMA; CRYSTAL-STRUCTURE; REPRESSOR; BINDING; DIFFRACTION; REFINEMENT; PROTEINS; HISTORY; FINGER; MODELS;

D O I：

10.1107/S1744309109025196

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member of the Pfam PF07336 family classified as a domain of unknown function (DUF1470), was solved to a resolution of 1.45 angstrom by multiple-wavelength anomalous dispersion (MAD). This protein is the first structural representative of the DUF1470 Pfam family. Structural analysis revealed a two-domain organization, with the N-terminal domain presenting a new fold called the ABATE domain that may bind an as yet unknown ligand. The C-terminal domain forms a treble-clef zinc finger that is likely to be involved in DNA binding. Analysis of the Jann_2411 protein and the broader ABATE-domain family suggests a role as stress-induced transcriptional regulators.

引用

页码：1198 / 1204

页数：7