Steady-state levels of vasoactive intestinal peptide (VIP) mRNA in goat ovaries and the effect of VIP on the in vitro development of isolated preantral follicles

被引：1
作者：
Bruno, J. B. [1 ]
Celestino, J. J. H. [2 ]
Saraiva, M. V. A. [1 ]
Rocha, R. M. P. [1 ]
Brito, I. R. [1 ]
Duarte, A. B. G. [1 ]
Araujo, V. R. [1 ]
Silva, C. M. G. [1 ]
Lima, I. M. T. [3 ]
Matos, M. H. T. [4 ]
Campello, C. C. [1 ]
Silva, J. R. V. [5 ]
Figueiredo, J. R. [1 ]
机构：
[1] Univ Estadual Ceara, PPGCV, LAMOFOPA, Fac Vet Med, Fortaleza, Ceara, Brazil
[2] Univ Int Integrat Lusophone African Brazilian, Inst Hlth Sci, Acarape, CE, Brazil
[3] Mauricio de Nassau Fac, Fortaleza, Ceara, Brazil
[4] Fed Univ Sao Francisco Valley, Nucleus Biotechnol Appl Ovarian Follicle Dev, Petrolina, PE, Brazil
[5] Univ Fed Ceara, Biotechnol Nucleus Sobral NUBIS, Sobral, CE, Brazil
来源：
ANIMAL REPRODUCTION | 2014年 / 11卷 / 04期
关键词：
caprine; culture; FSH; preantral follicles; VIP; STIMULATING-HORMONE RECEPTORS; RAT GRANULOSA-CELLS; CYCLASE-ACTIVATING POLYPEPTIDE; RIBONUCLEIC-ACID LEVELS; FSH RECEPTOR; STEROID-SECRETION; OOCYTE MATURATION; GENE-EXPRESSION; MOUSE OVARY; GROWTH;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The aims of this study were to verify the steady-state level of vasoactive intestinal peptide (VIP) mRNA in goat follicles at various developmental stages and to investigate the influence of VIP on the survival, antrum formation and growth of secondary follicles cultured for 6 days. Primordial, primary and secondary goat follicles and small and large antral follicles were obtained to quantify VIP mRNA by real-time reverse transcription with the polymerase chain reaction. The influence of VIP and the presence or absence of folliclestimulating hormone (FSH) on the development of secondary follicles and on mRNA expression for VIP and FSH receptor (FSHR) were determined after 6 days of culture. Survival, antrum formation and follicular diameter were evaluated every other day of culture. The levels of VIP mRNA in primary and secondary follicles were significantly higher than in primordial follicles. Cumulus-oocyte complexes (COCs) from both small and large antral follicles had significantly higher levels of VIP mRNA than their respective granulosa/theca cells. During culture, the addition of VIP and/or FSH had no effect on follicular development. However, the presence of FSH and/or VIP in the culture medium significantly reduced VIP mRNA levels, but did not alter FSHR mRNA levels. In conclusion, VIP mRNA was detected in all goat follicular categories and cellular types, VIP and/or FSH did not affect the development of secondary follicles and reduce the expression of VIP mRNA levels.
引用
收藏
页码：557 / 566
页数：10
相关论文
共 53 条
[1]   THE IMMATURE RAT OVARY IS INNERVATED BY VASOACTIVE-INTESTINAL-PEPTIDE (VIP)-CONTAINING FIBERS AND RESPONDS TO VIP WITH STEROID-SECRETION [J].
AHMED, CE ;
DEES, WL ;
OJEDA, SR .
ENDOCRINOLOGY, 1986, 118 (04) :1682-1689
[2]   Effect of pituitary adenylate cyclase-activating peptide on meiotic maturation in follicle-enclosed, cumulus-enclosed, and denuded rat oocytes [J].
Apa, R ;
Lanzone, A ;
Mastrandrea, M ;
Miceli, F ;
Macchione, E ;
Fulghesu, AM ;
Caruso, A ;
Canipari, R .
BIOLOGY OF REPRODUCTION, 1997, 57 (05) :1074-1079
[3]   Expression localisation and functional activity of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors in mouse ovary [J].
Barberi, Marzia ;
Muciaccia, Barbara ;
Morelli, Maria Beatrice ;
Stefanini, Mario ;
Cecconi, Sandra ;
Canipari, Rita .
REPRODUCTION, 2007, 134 (02) :281-292
[4]   Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture [J].
Bruno, J. B. ;
Celestino, J. J. H. ;
Lima-Verde, I. B. ;
Matos, M. H. T. ;
Lima, L. F. ;
Name, K. P. O. ;
Araujo, V. R. ;
Saraiva, M. V. A. ;
Martins, F. S. ;
Campello, C. C. ;
Silva, J. R. V. ;
Bao, S. N. ;
Figueiredo, J. R. .
CELLS TISSUES ORGANS, 2010, 191 (05) :414-421
[5]  
Burden H.W., 1985, CATECHOLAMINES HORMO, P261
[6]   In vitro development of sheep preantral follicles [J].
Cecconi, S ;
Barboni, B ;
Coccia, M ;
Mattioli, M .
BIOLOGY OF REPRODUCTION, 1999, 60 (03) :594-601
[7]   Effect of pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal polypeptide on mouse preantral follicle development in vitro [J].
Cecconi, S ;
Rossi, G ;
Barberi, M ;
Scaldaferri, L ;
Canipari, R .
ENDOCRINOLOGY, 2004, 145 (04) :2071-2079
[8]   Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro [J].
Chaves, R. N. ;
Martins, F. S. ;
Saraiva, M. V. A. ;
Celestino, J. J. H. ;
Lopes, C. A. P. ;
Correia, J. C. ;
Verde, I. B. Lima ;
Matos, M. H. T. ;
Bao, S. N. ;
Name, K. P. O. ;
Campello, C. C. ;
Silva, J. R. V. ;
Figueiredo, J. R. .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2008, 20 (05) :640-647
[9]   Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro [J].
Cortvrindt, R ;
Smitz, J ;
VanSteirteghem, AC .
HUMAN REPRODUCTION, 1997, 12 (04) :759-768
[10]   Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin [J].
Cortvrindt, RG ;
Hu, YX ;
Liu, J ;
Smitz, JEJ .
FERTILITY AND STERILITY, 1998, 70 (06) :1114-1125
123456