Steady-state levels of vasoactive intestinal peptide (VIP) mRNA in goat ovaries and the effect of VIP on the in vitro development of isolated preantral follicles

被引：1
|
作者：
Bruno, J. B. [1 ]
Celestino, J. J. H. [2 ]
Saraiva, M. V. A. [1 ]
Rocha, R. M. P. [1 ]
Brito, I. R. [1 ]
Duarte, A. B. G. [1 ]
Araujo, V. R. [1 ]
Silva, C. M. G. [1 ]
Lima, I. M. T. [3 ]
Matos, M. H. T. [4 ]
Campello, C. C. [1 ]
Silva, J. R. V. [5 ]
Figueiredo, J. R. [1 ]
机构：
[1] Univ Estadual Ceara, PPGCV, LAMOFOPA, Fac Vet Med, Fortaleza, Ceara, Brazil
[2] Univ Int Integrat Lusophone African Brazilian, Inst Hlth Sci, Acarape, CE, Brazil
[3] Mauricio de Nassau Fac, Fortaleza, Ceara, Brazil
[4] Fed Univ Sao Francisco Valley, Nucleus Biotechnol Appl Ovarian Follicle Dev, Petrolina, PE, Brazil
[5] Univ Fed Ceara, Biotechnol Nucleus Sobral NUBIS, Sobral, CE, Brazil
来源：
ANIMAL REPRODUCTION | 2014年 / 11卷 / 04期
关键词：
caprine; culture; FSH; preantral follicles; VIP; STIMULATING-HORMONE RECEPTORS; RAT GRANULOSA-CELLS; CYCLASE-ACTIVATING POLYPEPTIDE; RIBONUCLEIC-ACID LEVELS; FSH RECEPTOR; STEROID-SECRETION; OOCYTE MATURATION; GENE-EXPRESSION; MOUSE OVARY; GROWTH;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The aims of this study were to verify the steady-state level of vasoactive intestinal peptide (VIP) mRNA in goat follicles at various developmental stages and to investigate the influence of VIP on the survival, antrum formation and growth of secondary follicles cultured for 6 days. Primordial, primary and secondary goat follicles and small and large antral follicles were obtained to quantify VIP mRNA by real-time reverse transcription with the polymerase chain reaction. The influence of VIP and the presence or absence of folliclestimulating hormone (FSH) on the development of secondary follicles and on mRNA expression for VIP and FSH receptor (FSHR) were determined after 6 days of culture. Survival, antrum formation and follicular diameter were evaluated every other day of culture. The levels of VIP mRNA in primary and secondary follicles were significantly higher than in primordial follicles. Cumulus-oocyte complexes (COCs) from both small and large antral follicles had significantly higher levels of VIP mRNA than their respective granulosa/theca cells. During culture, the addition of VIP and/or FSH had no effect on follicular development. However, the presence of FSH and/or VIP in the culture medium significantly reduced VIP mRNA levels, but did not alter FSHR mRNA levels. In conclusion, VIP mRNA was detected in all goat follicular categories and cellular types, VIP and/or FSH did not affect the development of secondary follicles and reduce the expression of VIP mRNA levels.
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收藏
页码：557 / 566
页数：10
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