Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system

被引：1

作者：

Luo, Qian ^{[1
,2
,3
,4
]}

Wei, Cong ^{[1
,2
,3
,4
]}

Long, Yan ^{[1
,2
,3
,4
]}

Zhang, Meng ^{[1
,2
,3
,4
]}

Shan, Wei ^{[1
,2
,3
,4
]}

Li, Honghu ^{[1
,2
,3
,4
]}

Cai, Shuyang ^{[1
,2
,3
,4
]}

Xu, Yulin ^{[1
,2
,3
,4
]}

Qian, Pengxu ^{[1
,2
,3
,4
,5
,6
]}

Huang, He ^{[1
,2
,3
,4
,5
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, BoneMarrow Transplantat Ctr, Hangzhou 310012, Zhejiang, Peoples R China

[2] Zhejiang Univ, Inst Hematol, Hangzhou 310012, Zhejiang, Peoples R China

[3] Zhejiang Engn Lab Stem Cell & Immunotherapy, Hangzhou 310012, Zhejiang, Peoples R China

[4] Zhejiang Univ, Med Ctr, Zhejiang Lab Syst & Precis Med, Hangzhou 310012, Zhejiang, Peoples R China

[5] Zhejiang Univ, Sch Med, Ctr Stem Cell & Regenerat Med, Hangzhou 310012, Peoples R China

[6] Zhejiang Univ, Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Hangzhou 310012, Zhejiang, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 53卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102350

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.

引用

页数：4

共 3 条

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