BODIPY-NBD dyad for highly selective and sensitive detection of hydrogen sulfide in cells and zebrafish

被引：9

作者：

Xin, Yue ^{[1
]}

Wang, Mei ^{[1
]}

Liu, Mengfei ^{[1
]}

Chen, Yinuo ^{[1
]}

Zhao, Han ^{[1
]}

Zhang, Pingzhu ^{[1
]}

Li, Xiaoliu ^{[1
]}

Wei, Chao ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Chem Biol Hebei Prov, Key Lab Med Chem & Mol Diag,Minist Educ, Baoding 071002, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2022年 / 272卷

关键词：

Hydrogen sulfide; NBD; Fluorescent probe; Bioimaging; FLUORESCENT-PROBE; H2S SYNTHESIS; LIVING CELLS; THIOLYSIS; SPECTRA; AMINE;

D O I：

10.1016/j.saa.2022.121007

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Hydrogen sulfide (H2S) has been regarded as the third endogenous gas signaling molecule. The development of suitable tools for H2S detection in vitro and in vivo has always been a focus of research. In this work, three BODIPY-NBD dyads (o/m/p-BNP) were designed and synthesized using BODIPY and NBD as the fluorophore and quencher, respectively. The position of the NBD moiety in the probe showed different fluorescence quenching abilities. All probes showed highly selective to H2S. Probe o-BNP displayed the maximum fluorescence enhancement (c.a. 1300-fold) and the lowest detection limit (105 nM). Probe o-BNP can visualize the production of endogenous H2S in HeLa cells and zebrafish. (C) 2022 Elsevier B.V. All rights reserved.

引用

页数：6

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