Pregnancies achieved with testicular and ejaculated spermatozoa in combination with intracytoplasmic sperm injection in men with totally or initially immotile spermatozoa in the ejaculate

The efficacy of intracytoplasmic sperm injection (ICSI) employing testicular and ejaculated spermatozoa was assessed in 24 couples with totally or initially immotile spermatozoa, No criteria were employed in selecting which patients would be treated with testicular or ejaculated spermatozoa, The men were chosen at random, Testicular spermatozoa obtained by testicular sperm extraction were used in 14 and ejaculated spermatozoa were used in 10 of these couples, In all cases, asthenozoospermia was total in their basal semen sample, In 12 male partners, spermatozoa were totally immotile before and after Percoll gradient fractionation (totally immotile), In the remaining 12 men, spermatozoa initially showed a total absence of motility; however, some of the spermatozoa had showed very poor motility (0.1%) after Percoll gradient fractionation and a 1.5-2.0 h incubation period (initially immotile), Of these 24 total asthenozoospermic males, 14 also had total teratozoospermia, The fertilization and cleavage rates in the testicular and ejaculated sperm groups were 53.5 and 96.3 and 54.5 and 94.4% respectively, One cycle resulted in complete fertilization failure, and in 23 embryo transfer cycles a total of 10 pregnancies were obtained (41.6%), Eight pregnancies were achieved in the testicular sperm group, while only two pregnancies were obtained in the ejaculated sperm group, Four pregnancies, two from the ejaculated sperm group and two from the testicular sperm group, resulted in clinical abortions in the first trimester, Of the remaining six pregnancies, two have already resulted in healthy births and four pregnancies are now in the second or third trimester in the testicular sperm group, Using testicular spermatozoa in combination with ICSI can be an alternative mode of treatment in cases with totally or initially immotile spermatozoa in the ejaculate, Very low pregnancy rates have been obtained and no ongoing pregnancy has been achieved using ejaculated spermatozoa in these cases.