IκBζ controls NLRP3 inflammasome activation via upregulation of the Nlrp3 gene

被引：11

作者：

Kim, Jeongeun ^{[1
,2
]}

Ahn, Huijeong ^{[1
,2
]}

Yu, Sangjung ^{[1
,2
]}

Ahn, Jae-Hee ^{[3
]}

Ko, Hyun-Jeong ^{[3
]}

Kweon, Mi-Na ^{[4
]}

Hong, Eui-Ju ^{[5
,6
]}

An, Beum-Soo ^{[7
]}

Lee, Eunsong ^{[1
,2
]}

Lee, Geun-Shik ^{[1
,2
]}

机构：

[1] Kangwon Natl Univ, Coll Pharm, Coll Vet Med, Chunchon 24341, Gangwon, South Korea

[2] Kangwon Natl Univ, Coll Pharm, Inst Vet Sci, Chunchon 24341, Gangwon, South Korea

[3] Kangwon Natl Univ, Coll Pharm, Lab Microbiol & Immunol, Chunchon 24341, Gangwon, South Korea

[4] Univ Ulsan, Coll Med, Asan Med Ctr, Mucosal Immunol Lab,Dept Convergence Med, Seoul 05505, South Korea

[5] Chungnam Natl Univ, Coll Vet Med, Daejeon 34134, South Korea

[6] Chungnam Natl Univ, Inst Vet Sci, Daejeon 34134, South Korea

[7] Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Biomat Sci, Gyeongsangnam Do 50612, South Korea

来源：

CYTOKINE | 2020年 / 127卷

基金：

新加坡国家研究基金会;

关键词：

Inflammasome; I kappa B zeta; Nfkbiz; Macrophages; Priming step; INHIBITS NLRP3; AIM2; EXPRESSION; PROTEIN; NLRC4; ACID;

D O I：

10.1016/j.cyto.2019.154983

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inflammasome activation induces the maturation and secretion of interleukin (IL)-1 beta and -18, and is dependent on NF-kappa B signaling to induce the transcription of the inflammasome components, called the priming step. This study elucidated the role of I kappa B zeta, an atypical l kappa Bs (inhibitor of kappa B) and a coactivator of NF-kappa B target genes, on the activation of inflammasome. Bone marrow-derived macrophages (BMDMs) that originated from I kappa B zeta-encoding Nfkbiz gene depletion mice presented a defect in NLRP3 inflammasome activation. In addition, the Nfkbiz(+/-) and Nfkbiz(-/-) mice significantly attenuated serum IL-1 beta secretion in response to a monosodium urate injection, a NLRP3 trigger, when compared with Nfkbiz(-/-) mice. The lack of I kappa B zeta in BMDMs produced a disability in the expression of Nlrp3 and pro-il1 beta mRNAs during the priming step. In addition, ectopic I kappa B zeta expression enhanced the Nlrp3 promoter activity, and Nlrp3 and pro-il1 beta transcription. Overall, I kappa B zeta controlled the activation of NLRP3 inflammasome by upregulating the Nlrp3 gene during the priming step.

引用

页数：8

共 35 条

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