Do zwitterions contribute to the ionic strength of a solution?

被引：45

作者：

Stellwagen, Earle ^{[1
]}

Prantner, Jason D. ^{[1
]}

Stellwagen, Nancy C. ^{[1
]}

机构：

[1] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 373卷 / 02期

关键词：

APPARENT MOLAR VOLUMES; CAPILLARY-ELECTROPHORESIS; INTERACTION CHROMATOGRAPHY; QUANTITATIVE-ANALYSIS; COUNTERION BINDING; DNA; BUFFERS; NACL; MOBILITY; GLYCINE;

D O I：

10.1016/j.ab.2007.10.038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis has been used to determine whether zwitterions contribute to the ionic strength of a solution, by measuring the mobility of a double-stranded DNA oligomer in cacodylate-buffered solutions containing various concentrations of the ionic salt tetraethylammonium chloride (TEA(+)Cl(-)) or the zwitterion tricine(+/-). The mobility of the DNA decreased as the square root of ionic strength, as expected from the Debye-Huckel-Onsager theory of electrophoresis, when TEA+Cl- was added to the buffer. However, the mobility was independent of the concentration of added tricine(+/-). Hence, zwitterions do not contribute to the ionic strength of a solution. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：407 / 409

页数：3

共 30 条

[1] VISCOSITIES AND APPARENT MOLAR VOLUMES OF SOME AMINO-ACIDS IN WATER AND IN 6M GUANIDINE-HYDROCHLORIDE AT 25-DEGREES-C [J].