Identification of Lhcb1/Lhcb2/Lhcb3 heterotrimers of the main light-harvesting chlorophyll a/b-protein complex of Photosystem II (LHC II)

被引:52
|
作者
Jackowski, G
Kacprzak, K
Jansson, S
机构
[1] Adam Mickiewicz Univ, Dept Plant Physiol, PL-61713 Poznan, Poland
[2] Adam Mickiewicz Univ, Dept Organ Stereochem, PL-60780 Poznan, Poland
[3] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
来源
关键词
light harvesting complex II; subcomplex; trimer; (Arabidopsis thaliana);
D O I
10.1016/S0005-2728(00)00262-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using non-denaturing isoelectric focusing in polyacrylamide vertical slab gel, we have purified to homogeneity three trimeric subcomplexes of LHC II from Arabidopsis thylakoid membranes. The polypeptide composition of the subcomplexes were studied by immunoblotting. Our results indicate the existence in vivo of LHC II heterotrimers containing Lhcb1, Lhcb2 and Lhcb3 gene products. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
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页码:340 / 345
页数:6
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