Biotransformation of Flurbiprofen by Cunninghamella Species

被引：36
作者：
Amadio, Jessica [1 ]
Gordon, Katherine [1 ]
Murphy, Cormac D. [1 ]
机构：
[1] Natl Univ Ireland Univ Coll Dublin, Sch Biomol & Biomed Sci, Ctr Synth & Chem Biol, Dublin 4, Ireland
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 18期
关键词：
MAMMALIAN METABOLISM; CYTOCHROME-P450; 2C9; MICROBIAL MODELS; DRUG-METABOLISM; TRANSFORMATION; ELEGANS; 4'-HYDROXYLATION; CHROMATOGRAPHY; URINE;
D O I：
10.1128/AEM.01027-10
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The biotransformation of the fluorinated anti-inflammatory drug flurbiprofen was investigated in Cunninghamella spp. Mono- and dihydroxylated metabolites were detected using gas chromatography-mass spectrometry and fluorine-19 nuclear magnetic resonance spectroscopy, and the major metabolite 4'-hydroxyflurbiprofen was isolated by preparative high-pressure liquid chromatography (HPLC). Cunninghamella elegans DSM 1908 and C. blakesleeana DSM 1906 also produced a phase II (conjugated) metabolite, which was identified as the sulfated drug via deconjugation experiments.
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页码：6299 / 6303
页数：5
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