Unravelling biology and shifting paradigms in cancer with single-cell sequencing

被引：302

作者：

Baslan, Timour ^{[1
,2
]}

Hicks, James ^{[3
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Canc Biol & Genet Program, New York, NY 10044 USA

[2] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA

[3] Univ Southern Calif, Dana & David Dornsife Coll Letters Arts & Sci, Los Angeles, CA 90089 USA

来源：

NATURE REVIEWS CANCER | 2017年 / 17卷 / 09期

关键词：

HEALTH-ORGANIZATION CLASSIFICATION; CIRCULATING TUMOR-CELLS; FINE-NEEDLE-ASPIRATION; GENOME-WIDE DETECTION; MESSENGER-RNA-SEQ; CHROMATIN ACCESSIBILITY; ACQUIRED-RESISTANCE; BARRETTS-ESOPHAGUS; GENE-EXPRESSION; LUNG-CANCER;

D O I：

10.1038/nrc.2017.58

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The fundamental operative unit of a cancer is the genetically and epigenetically innovative single cell. Whether proliferating or quiescent, in the primary tumour mass or disseminated elsewhere, single cells govern the parameters that dictate all facets of the biology of cancer. Thus, single-cell analyses provide the ultimate level of resolution in our quest for a fundamental understanding of this disease. Historically, this quest has been hampered by technological shortcomings. In this Opinion article, we argue that the rapidly evolving field of single-cell sequencing has unshackled the cancer research community of these shortcomings. From furthering an elemental understanding of intra-tumoural genetic heterogeneity and cancer genome evolution to illuminating the governing principles of disease relapse and metastasis, we posit that single-cell sequencing promises to unravel the biology of all facets of this disease.

引用

页码：557 / 569

页数：13

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