Negative regulation of melatonin secretion by melatonin receptors in ovine pinealocytes

被引:4
|
作者
Lepinay, Julie [1 ]
Taragnat, Catherine [1 ]
Dubois, Jean-Philippe [1 ]
Chesneau, Didier [1 ]
Jockers, Ralf [2 ]
Delagrange, Philippe [3 ]
Bozon, Veronique [1 ]
机构
[1] Univ Tours, Physiol Reprod & Comportements, Nouzilly, France
[2] Univ Paris, CNRS, Inst Cochin, INSERM, Paris, France
[3] Inst Rech Servier, Croissy Sur Seine, France
来源
PLOS ONE | 2021年 / 16卷 / 07期
关键词
ARYLALKYLAMINE N-ACETYLTRANSFERASE; PARS-TUBERALIS; MOLECULAR PHARMACOLOGY; SIGNAL-TRANSDUCTION; CALCIUM-CHANNELS; SERUM MELATONIN; BINDING-SITES; MT1; ASTROCYTES; CELLS;
D O I
10.1371/journal.pone.0255249
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Melatonin (MLT) is a biological modulator of circadian and seasonal rhythms and reproduction. The photoperiodic information is detected by retinal photoreceptors and transmitted through nerve transmissions to the pineal gland, where MLT is synthesized and secreted at night into the blood. MLT interacts with two G protein-coupled receptors, MT1 and MT2. The aim of our work was to provide evidence for the presence of MLT receptors in the ovine pineal gland and define their involvement on melatonin secretion. For the first time, we identified the expression of MLT receptors with the specific 2-[I-125]-MLT agonistic radioligand in ovin pinealocytes. The values of Kd and Bmax are 2.24 +/- 1.1 nM and 20 +/- 6.8 fmol/mg. MLT receptors are functional and inhibit cAMP production and activate ERK1/2 through pertussis toxin-sensitive G(i/o) proteins. The MLT receptor antagonist/ inverse agonist luzindole increased cAMP production (189 +/- 30%) and MLT secretion (866 +/- 13%). The effect of luzindole on MLT secretion was additive with the effect of well-described activators of this pathway such as the beta-adrenergic agonist isoproterenol and the alpha-adrenergic agonist phenylephrine. Co-incubation of all three compounds increased MLT secretion by 1236 +/- 199%. These results suggest that MLT receptors are involved in the negative regulation of the synthesis of its own ligand in pinealocytes. While adrenergic receptors promote MLT secretion, MLT receptors mitigate this effect to limit the quantity of MLT secreted by the pineal gland.
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页数:20
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