Tissue culture optimization for Lallementia royleana L. an important medicinal plant

被引：1

作者：

Mahmood, Tariq ^{[1
]}

Arshad, Huma ^{[1
]}

Akhtar, Wasim ^{[2
]}

Ilyas, Muhammad ^{[3
]}

Habib, Tariq ^{[2
]}

Shinwari, Zabta K. ^{[1
]}

机构：

[1] Quaid I Azam Univ, Dept Plant Sci, Islamabad, Pakistan

[2] Univ Azad Jammu & Kashmir Muzaffarabad, Dept Bot, Muzaffarabad, Pakistan

[3] Univ Swabi KP, Dept Bot, Swabi, Pakistan

来源：

IHERINGIA SERIE BOTANICA | 2021年 / 76卷

关键词：

in vitro culture; micropropagation; shooting; IN-VITRO PROPAGATION; SOMATIC EMBRYOGENESIS; CLONAL PROPAGATION; CALLUS-CULTURES; LEAF EXPLANTS; REGENERATION; INDUCTION; SHOOT; LAMIACEAE; GROWTH;

D O I：

10.21826/2446-82312021v76e2021009

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.

引用

页数：11

共 44 条

[31] The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica [J].