Saliva for molecular detection of SARS-CoV-2 in pre-school and school-age children

被引：3

作者：

Delaunay-Moisan, Agnes ^{[1
]}

Guilleminot, Tiffany ^{[2
]}

Semeraro, Michaela ^{[3
]}

Briand, Nelly ^{[3
]}

Bader-Meunier, Brigitte ^{[4
,5
]}

Berthaud, Romain ^{[3
]}

Morelle, Guillaume ^{[6
]}

Quartier, Pierre ^{[4
]}

Galeotti, Caroline ^{[6
]}

Basmaci, Romain ^{[7
]}

Benoist, Gregoire ^{[8
]}

Gajdos, Vincent ^{[9
]}

Lorrot, Mathie ^{[10
]}

Rifai, Mahmoud ^{[11
]}

Crespin, Matis ^{[11
]}

M'Sakni, Zakary ^{[11
]}

Padavia, Faheemah ^{[3
]}

Savetier-Leroy, Catherine ^{[12
]}

Lorenzi, Michelle ^{[12
]}

Maurin, Caroline ^{[12
]}

Behillil, Sylvie ^{[13
]}

de Pontual, Loic ^{[14
]}

Elenga, Narcisse ^{[15
]}

Bouazza, Naim ^{[3
]}

Moltrecht, Brigitte ^{[12
]}

van der Werf, Sylvie ^{[13
]}

Leruez-Ville, Marianne ^{[2
]}

Sermet-Gaudelus, Isabelle ^{[11
,16
,17
]}

机构：

[1] Univ Paris Saclay, CEA, CNRS, Inst Integrat Biol Cell I2BC, Gif Sur Yvette, France

[2] Hosp Necker Enfants Malad, Assistance Publ Hop Paris, UMR 7328 Imagine Paris Cite, Virol Lab, Paris, France

[3] Hop Necker Enfants Malad, Assistance Publ Hop Paris, Unite Rech Clin, Ctr Invest Clin, Paris, France

[4] Necker Enfants Malad Hosp, AP HP, Dept Paediat Hematol Immunol & Rheumatol, Paris, France

[5] Imagine Inst, Reference Ctr Rheumat AutoImmune & Syst Dis Child, Inserm U1163, Paris, France

[6] Hop Kremlin Bicetre, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[7] Hop Louis Mourier, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[8] Hop Ambroise Pare, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[9] Hop Antoine Beclere, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[10] Hop Armand Trousseau, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[11] CHU Necker Enfants Malad, Assistance Publ Hop Paris, Ctr References Malad Rares Mucoviscidose & Malad, F-75015 Paris, France

[12] Minist Educ Natl, Paris, France

[13] Inst Pasteur, Ctr Reference Virus Emergents, Paris, France

[14] Univ Sorbonne Paris Nord, Hop Jean Verdier, Assistance Publ Hop Paris, Serv Pediat Gen, Paris, France

[15] Ctr Hosp Cayenne Andree Rosemon, Serv Pediat Gen, Cayenne, France

[16] Univ Paris Cite, Inst Necker Enfants Malad, INSERM UMR SU1151, Paris, France

[17] European Reference Network Rare Resp Dis, Brussels, Belgium

来源：

ENVIRONMENTAL MICROBIOLOGY | 2022年 / 24卷 / 10期

关键词：

D O I：

10.1111/1462-2920.16151

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

SARS-CoV-2 diagnosis is a cornerstone for the management of coronavirus disease 2019 (COVID-19). Numerous studies have assessed saliva performance over nasopharyngeal sampling (NPS), but data in young children are still rare. We explored saliva performance for SARS-CoV-2 detection by RT-PCR according to the time interval from initial symptoms or patient serological status. We collected 509 NPS and saliva paired samples at initial diagnosis from 166 children under 12 years of age (including 57 children under 6), 106 between 12 and 17, and 237 adults. In children under 12, overall detection rate for SARS-CoV-2 was comparable in saliva and NPS, with an overall agreement of 89.8%. Saliva sensitivity was significantly lower than that of NPS (77.1% compared to 95.8%) in pre-school and school-age children but regained 96% when considering seronegative children only. This pattern was also observed to a lesser degree in adolescents but not in adults. Sensitivity of saliva was independent of symptoms, in contrary to NPS, whose sensitivity decreased significantly in asymptomatic subjects. Performance of saliva is excellent in children under 12 at early stages of infection. This reinforces saliva as a collection method for early and unbiased SARS-CoV-2 detection and a less invasive alternative for young children.

引用

页码：4725 / 4737

页数：13

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