Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions

被引：96

作者：

Ge, Yun ^{[1
]}

Chen, Long ^{[1
]}

Liu, Shibo ^{[1
]}

Zhao, Jingyi ^{[1
]}

Zhang, Heng ^{[1
]}

Chen, Peng R. ^{[1
,2
]}

机构：

[1] Peking Univ, Synthet & Funct Biomol Ctr, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Beijing Natl Lab Mol Sci,Coll Chem &, Beijing, Peoples R China

[2] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2019年 / 141卷 / 05期

基金：

中国国家自然科学基金;

关键词：

BIOTIN LIGASE; PROTEINS; PEROXIDASE; YEAST; TOOLS;

D O I：

10.1021/jacs.8b10286

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cell-cell interactions and communications play fundamental roles in life processes but remain largely uncharacterized. We developed an enzyme-mediated proximity cell labeling (EXCELL) strategy as a general method to detect and record cell-cell interactions under living conditions. EXCELL relies on an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA) capable of promiscuous labeling of various cell surface proteins containing a monoglycine residue at the N-terminus. Displaying mgSrtA on the surface of a cell of interest allows the labeling and detection of interacting cells in a proximity-dependent fashion.

引用

收藏

页码：1833 / 1837

页数：5

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