Micropropagation of Heracleum candicans Wall:: A rare medicinal herb

A protocol was developed for micropropagation of Heracleum candicans Wall. by axillary shoot proliferation. Maximum shoot proliferation was obtained on Murashige and Skoog medium supplemented with 0.5 mg (2.2 mu M) 6-benzyladenine per l and 0.1 mg (0.4 mu M) 1-naphthaleneacetic acid per l. Regenerated shoots were rooted on MS medium fortified with 1 mg (4.9 mu M) indole-3-butyric acid per l. Complete plants were transferred to soil and all of these plants were morphologically and cytologically identical to the mother plant.