Influence of sugar moiety of ovoglycoprotein on chiral discrimination

被引：7

作者：

Haginaka, J ^{[1
]}

Matsunaga, H ^{[1
]}

机构：

[1] Mukogawa Womens Univ, Fac Pharmaceut Sci, Nishinomiya, Hyogo 6638179, Japan

来源：

ANALYTICAL COMMUNICATIONS | 1999年 / 36卷 / 02期

关键词：

D O I：

10.1039/a808472f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The influence of sugar moieties of ovoglycoprotein from chicken egg whites (OGCHI) on chiral discrimination of various solutes has been investigated. Partially deglycosylated OGCHI and completely deglycosylated OGCHI were obtained by treatment of OGCHI with N-glycosidase, and endoglycosidase and N-glycosidase, respectively. The average molecular weights of OGCHI, partially deglycosylated OGCHI and completely deglycosylated OGCHI were estimated to be about 30 400, 28 200 and 21 700, respectively, by matrix-assisted laser desorption time-of-flight mass spectrometry. It was found that partially deglycosylated OGCHI showed excellent chiral recognition abilities comparable to OGCHI, and that completely deglycosylated OGCHI still showed chiral recognition abilities for various solutes tested. These results reveal that the chiral recognition site(s) for OGCHI is present on the protein domain of OGCHI.

引用

页码：39 / 41

页数：3

共 21 条

[1]

ALLENMARK S, 1991, CHROMATOGRAPHIC ENAN, P129

[2]

BRUNNER F, 1985, J PHARM PHARMACOL, V37, P30

[3] CHIRAL SEPARATION OF PROPRANOLOL AND ITS ESTER DERIVATIVES ON AN OVOMUCOID-BONDED SILICA - INFLUENCE OF PH, IONIC-STRENGTH AND ORGANIC MODIFIER ON RETENTION, ENANTIOSELECTIVITY AND ENANTIOMERIC ELUTION ORDER [J].