Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens

被引：61
作者：
Hwang, Seon-Hee [2 ]
Yie, Se Won [2 ]
Hwang, Duk-Ju [1 ]
机构：
[1] Rural Dev Adm, Natl Acad Agr Sci, Biocrop Dev Div, Suwon 440707, South Korea
[2] Kangwon Natl Univ, Sch Biosci & Biotechnol, Dept Mol Biosci, Chunchon 200701, South Korea
来源：
PLANT SCIENCE | 2011年 / 181卷 / 03期
关键词：
WRKY transcription factor; Disease resistance; WRKY TRANSCRIPTION FACTORS; DNA-BINDING PROTEINS; DISEASE RESISTANCE; SIGNALING PATHWAY; RICE; SUPERFAMILY; FAMILY; PROMOTER; ELEMENTS; GROWTH;
D O I：
10.1016/j.plantsci.2011.06.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The WRKY proteins are a major family of plant transcription factors implicated in the regulation of plant defense mechanisms against pathogens. OsWRKY6 was isolated based on expression profiling data carried out with samples infected by Xanthomonas oryzae pv. oryzae (Xoo). OsWRKY6 encodes a DNA binding protein that contains one WRKY domain, a nuclear localization signal and C2H2-type zinc finger motif. OsWRKY6 is a member of the group II family of WRKY proteins. Based on the result of yeast one hybrid assay this OsWRKY6 protein binds to the typical W box ((T)TGACC/T). OsWRKY6 functions as a transcriptional activator in yeast. OsWRKY6 enhanced the expression of the reporter gene downstream of OsPR1 promoter, indicating that OsWRKY6 is a transcriptional activator in rice as well. Heterologous expression of OsWRKY6 enhanced disease resistance to pathogen. Defense-related genes were constitutively expressed in Arabidopsis transgenic lines overexpressing OsWRKY6. All together, OsWRKY6 functions as a positive transcriptional regulator of the plant defense response. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：316 / 323
页数：8
相关论文
共 53 条
  • [1] MAP kinase signalling cascade in Arabidopsis innate immunity
    Asai, T
    Tena, G
    Plotnikova, J
    Willmann, MR
    Chiu, WL
    Gomez-Gomez, L
    Boller, T
    Ausubel, FM
    Sheen, J
    [J]. NATURE, 2002, 415 (6875) : 977 - 983
  • [2] Characterization of WRKY co-regulatory networks in rice and Arabidopsis
    Berri, Stefano
    Abbruscato, Pamela
    Faivre-Rampant, Odile
    Brasileiro, Ana C. M.
    Fumasoni, Irene
    Satoh, Kouji
    Kikuchi, Shoshi
    Mizzi, Luca
    Morandini, Piero
    Pe, Mario Enrico
    Piffanelli, Pietro
    [J]. BMC PLANT BIOLOGY, 2009, 9
  • [3] WRKY72-type transcription factors contribute to basal immunity in tomato and Arabidopsis as well as gene-for-gene resistance mediated by the tomato R gene Mi-1
    Bhattarai, Kishor K.
    Atamian, Hagop S.
    Kaloshian, Isgouhi
    Eulgem, Thomas
    [J]. PLANT JOURNAL, 2010, 63 (02) : 229 - 240
  • [4] Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor
    Chen, CH
    Chen, ZX
    [J]. PLANT PHYSIOLOGY, 2002, 129 (02) : 706 - 716
  • [5] Isolation and characterization of two pathogen- and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco
    Chen, CH
    Chen, ZX
    [J]. PLANT MOLECULAR BIOLOGY, 2000, 42 (02) : 387 - 396
  • [6] Comparative analyses of genomic locations and race specificities of loci for quantitative resistance to Pyricularia grisea in rice and barley
    Chen, H
    Wang, SP
    Xing, YZ
    Xu, CG
    Hayes, PM
    Zhang, QF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) : 2544 - 2549
  • [7] Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light
    Chern, M
    Fitzgerald, HA
    Canlas, PE
    Navarre, DA
    Ronald, PC
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2005, 18 (06) : 511 - 520
  • [8] Chuio T., 2009, BIOSCI BIOTECH BIOCH, V73, P1901
  • [9] Characterization of the novel gene BjDREB1B encoding a DRE-binding transcription factor from Brassica juncea L.
    Cong, Lin
    Chai, Tuan-Yao
    Zhang, Yu-Xiu
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 371 (04) : 702 - 706
  • [10] A potato gene encoding a WRKY-like transcription factor is induced in interactions with Erwinia carotovora subsp atroseptica and Phytophthora infestans and is coregulated with class I endochitinase expression
    Dellagi, A
    Heilbronn, J
    Avrova, AO
    Montesano, M
    Palva, ET
    Stewart, HE
    Toth, IK
    Cooke, DEL
    Lyon, GD
    Birch, PRJ
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (10) : 1092 - 1101
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