Monoclonal antibody-based ELISA for the quantification of porcine hemoglobin in meat products

被引:30
作者
Jiang, Xingyi [1 ]
Fuller, Danielle [1 ]
Hsieh, Yun-Hwa Peggy [1 ]
Rao, Qinchun [1 ]
机构
[1] Florida State Univ, Dept Nutr Food & Exercise Sci, Tallahassee, FL 32306 USA
基金
美国食品与农业研究所;
关键词
Porcine hemoglobin; Adulteration; Affinity; Thermostability; Immunodetection; Food quality; IRON-DEFICIENCY; PROTEINS; BLOOD; ABSORPTION; AFFINITY; PIGLETS;
D O I
10.1016/j.foodchem.2018.01.032
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Misusage of porcine blood proteins, such as misbranding and substitution, can cause religious objections, law violation, and food quality concerns. These issues highlight the need for detecting unlabeled or overuse of porcine blood in foods. Compared with acidic and neutral pHs, porcine hemoglobin (P-Hb) at alkaline pH retained the best solubility, molecular integrity, and immunoreactivity after heat treatment. PHb at acidic and alkaline pHs remained stable during storage at 4 degrees C for 29 days. A monoclonal antibody (mAb) specific to mammalian hemoglobin, 13F7, was developed. A mAb13F7-based indirect competitive ELISA (icELISA) was optimized for the quantification of PHb in meat products. This assay had a wide working range from 0.5 ppm to 1000 ppm. It was sensitive (limit of detection: 0.5 ppm), precise and reproducible with low inter- and intra-coefficient of variances (< 20%). This assay is suitable for government, food industry, and third-party authority to surveillance food quality.
引用
收藏
页码:170 / 179
页数:10
相关论文
共 39 条
[1]  
Alina A. R., 2012, World Applied Sciences Journal, V17, P39
[2]  
[Anonymous], 2015, DIS PORK
[3]  
[Anonymous], 2010, COMP SAFE PORK DIS P
[4]  
[Anonymous], 1 PINK SLIME NOW MEA
[5]  
[Anonymous], 2012, Daily Mail Reporter
[6]  
[Anonymous], 2017, LIV POULTR WORLD MAR
[7]   Slaughterhouse Blood: An Emerging Source of Bioactive Compounds [J].
Bah, Clara S. F. ;
Bekhit, Alaa El-Din A. ;
Carne, Alan ;
McConnell, Michelle A. .
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2013, 12 (03) :314-331
[8]   UniProt: the universal protein knowledgebase [J].
Bateman, Alex ;
Martin, Maria Jesus ;
O'Donovan, Claire ;
Magrane, Michele ;
Alpi, Emanuele ;
Antunes, Ricardo ;
Bely, Benoit ;
Bingley, Mark ;
Bonilla, Carlos ;
Britto, Ramona ;
Bursteinas, Borisas ;
Bye-A-Jee, Hema ;
Cowley, Andrew ;
Da Silva, Alan ;
De Giorgi, Maurizio ;
Dogan, Tunca ;
Fazzini, Francesco ;
Castro, Leyla Garcia ;
Figueira, Luis ;
Garmiri, Penelope ;
Georghiou, George ;
Gonzalez, Daniel ;
Hatton-Ellis, Emma ;
Li, Weizhong ;
Liu, Wudong ;
Lopez, Rodrigo ;
Luo, Jie ;
Lussi, Yvonne ;
MacDougall, Alistair ;
Nightingale, Andrew ;
Palka, Barbara ;
Pichler, Klemens ;
Poggioli, Diego ;
Pundir, Sangya ;
Pureza, Luis ;
Qi, Guoying ;
Rosanoff, Steven ;
Saidi, Rabie ;
Sawford, Tony ;
Shypitsyna, Aleksandra ;
Speretta, Elena ;
Turner, Edward ;
Tyagi, Nidhi ;
Volynkin, Vladimir ;
Wardell, Tony ;
Warner, Kate ;
Watkins, Xavier ;
Zaru, Rossana ;
Zellner, Hermann ;
Xenarios, Ioannis .
NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D158-D169
[9]   MEASUREMENT OF MONOCLONAL-ANTIBODY AFFINITY BY NONCOMPETITIVE ENZYME-IMMUNOASSAY [J].
BEATTY, JD ;
BEATTY, BG ;
VLAHOS, WG .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 100 (1-2) :173-179
[10]  
Bhattarai S, 2015, J SWINE HEALTH PROD, V23, P10