Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients

被引：230

作者：

Casagrande, Maria ^{[1
]}

Fitzek, Antonia ^{[2
]}

Pueschel, Klaus ^{[2
]}

Aleshcheva, Ganna ^{[3
]}

Schultheiss, Heinz-Peter ^{[3
]}

Berneking, Laura ^{[4
]}

Spitzer, Martin S. ^{[1
]}

Schultheiss, Maximilian ^{[1
]}

机构：

[1] Univ Med Ctr Hamburg Eppendorf UKE, Dept Ophthalmol, Hamburg, Germany

[2] Univ Med Ctr Hamburg Eppendorf UKE, Inst Forens Med, Hamburg, Germany

[3] Inst Cardiac Diagnost & Therapy, Berlin, Germany

[4] Med Ctr Hamburg Eppendorf UKE, Inst Med Microbiol Virol & Hyg, Hamburg, Germany

来源：

OCULAR IMMUNOLOGY AND INFLAMMATION | 2020年 / 28卷 / 05期

关键词：

ACE-2; autopsy; COVID-19; retina; RT-PCR; SARS-CoV-2;

D O I：

10.1080/09273948.2020.1770301

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19). Patients and Methods Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater (TM). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2. Results In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization. Conclusion Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.

引用

页码：721 / 725

页数：5

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