Detection of genetic variation in Taenia solium

被引：34
作者：
Maravilla, P
Souza, V
Valera, A
Romero-Valdovinos, M
Lopez-Vidal, Y
Dominguez-Alpizar, JL
Ambrosio, J
Kawa, S
Flisser, A [1 ]
机构：
[1] SSA, Hosp Gen Dr Manuel Gea Gonzalez, Direcc Invest, Mexico City 11400, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
[3] Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Mexico City 04510, DF, Mexico
[4] Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Merida 97100, Yucatan, Mexico
来源：
JOURNAL OF PARASITOLOGY | 2003年 / 89卷 / 06期
关键词：
D O I：
10.1645/GE-2786RN
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Genetic variability among Taenia solium isolates was studied in 160 cysticerci from 6 pigs, 4 from Mexico, 1 from Honduras, and 1 from Tanzania. Random amplified polymorphic DNA (RAPD) analysis performed with 4 commercial primers showed 88% polymorphic loci and an average heterozygosity of 0.077; however, several alleles were fixed within each isolate. Linkage disequilibrium analysis indicated that 3 of the 6 isolates had a random association of alleles, whereas the other 3 had a clonal structure. These results suggest the existence of local lineages in T. solium, with events of genetic recombination within them.
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页码：1250 / 1254
页数：5
相关论文
共 22 条
[1]   GENETIC-CHARACTERIZATION OF THE ASIAN TAENIA, A NEWLY DESCRIBED TAENIID CESTODE OF HUMANS [J].
BOWLES, J ;
MCMANUS, DP .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1994, 50 (01) :33-44
[2]  
Brouwer KC, 2001, J PARASITOL, V87, P762, DOI 10.1645/0022-3395(2001)087[0762:GDOAPO]2.0.CO
[3]  
2
[4]  
Eom KS, 2002, J PARASITOL, V88, P758, DOI 10.1645/0022-3395(2002)088[0758:IOTAIC]2.0.CO
[5]  
2
[6]   Aedes (Finlaya) japonicus (Diptera: Culicidae), a newly recognized mosquito in the United States:: Analyses of genetic variation in the United States and putative source populations [J].
Fonseca, DM ;
Campbell, S ;
Crans, WJ ;
Mogi, M ;
Miyagi, I ;
Toma, T ;
Bullians, M ;
Andreadis, TG ;
Berry, RL ;
Pagac, B ;
Sardelis, MR ;
Wilkerson, RC .
JOURNAL OF MEDICAL ENTOMOLOGY, 2001, 38 (02) :135-146
[7]   Applications of random amplified polymorphic DNA (RAPD) in molecular ecology [J].
Hadrys, H. ;
Balick, M. ;
Schierwater, B. .
MOLECULAR ECOLOGY, 1992, 1 (01) :55-63
[8]   Sequence variation in the cytochrome oxidase I, internal transcribed spacer 1, and Ts14 diagnostic antigen sequences of Taenia solium isolates from South and Central America, India, and Asia [J].
Hancock, K ;
Broughel, DE ;
Moura, INS ;
Khan, A ;
Pieniazek, NJ ;
Gonzalez, AE ;
Garcia, HH ;
Gilman, RH ;
Tsang, VCW .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2001, 31 (14) :1601-1607
[9]   Intraspecific variability of Proteocephalus exiguus La Rue, 1911 (Cestoda: Proteocephalidae) as studied by the random amplified polymorphic DNA method [J].
Kralova, I ;
Spakulova, M .
PARASITOLOGY RESEARCH, 1996, 82 (06) :542-545
[10]   A total DNA characterization in Proteocephalus exiguus and P-percae (Cestoda: Proteocephalidae): Random amplified polymorphic DNA and hybridization techniques [J].
Kralova, I .
PARASITOLOGY RESEARCH, 1996, 82 (08) :668-671
123