Structural basis of signal-sequence recognition by the signal recognition particle

被引：67

作者：

Hainzl, Tobias ^{[1
]}

Huang, Shenghua ^{[1
]}

Merilainen, Gitte ^{[1
]}

Brannstrom, Kristoffer ^{[2
]}

Sauer-Eriksson, A. Elisabeth ^{[1
]}

机构：

[1] Umea Univ, Dept Chem, Umea, Sweden

[2] Umea Univ, Dept Med Biochem & Biophys, S-90187 Umea, Sweden

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 03期

基金：

瑞典研究理事会;

关键词：

SRP RNA; CRYSTAL-STRUCTURE; GTPASE DOMAIN; 4.5S RNA; RECEPTOR; COMPLEX; PROTEINS; RIBOSOME; BINDING; CORE;

D O I：

10.1038/nsmb.1994

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The signal recognition particle (SRP) recognizes and binds the signal sequence of nascent proteins as they emerge from the ribosome. We present here the 3.0-angstrom structure of a signal sequence bound to the Methanococcus jannaschii SRP core. Structural comparison with the free SRP core shows that signal-sequence binding induces formation of the GM-linker helix and a 180 degrees flip of the NG domain-structural changes that ensure a hierarchical succession of events during protein targeting.

引用

收藏

页码：389 / 391

页数：3

相关论文

共 18 条

[1] Crystal structure of the ribonucleoprotein core of the signal recognition particle [J].

Batey, RT ;

Rambo, RP ;

Lucast, L ;

Rha, B ;

Doudna, JA .

SCIENCE, 2000, 287 (5456) :1232-+

[2] Signal Sequences Activate the Catalytic Switch of SRP RNA [J].

Bradshaw, Niels ;

Neher, Saskia B. ;

Booth, David S. ;

Walter, Peter .

SCIENCE, 2009, 323 (5910) :127-130

[3] Substrate twinning activates the signal recognition particle and its receptor [J].

Egea, PF ;

Shan, SO ;

Napetschnig, J ;

Savage, DF ;

Walter, P ;

Stroud, RM .

NATURE, 2004, 427 (6971) :215-221

[4] Targeting proteins to membranes: structure of the signal recognition particle [J].

Egea, PF ;

Stroud, RM ;

Walter, P .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2005, 15 (02) :213-220

[5] Heterodimeric GTPase core of the SRP targeting complex [J].

Focia, PJ ;

Shepotinovskaya, IV ;

Seidler, JA ;

Freymann, DM .

SCIENCE, 2004, 303 (5656) :373-377

[6] Structure of the conserved GTPase domain of the signal recognition particle [J].

Freymann, DM ;

Keenan, RJ ;

Stroud, RM ;

Walter, P .

NATURE, 1997, 385 (6614) :361-364

[7] Structure of the SRP19-RNA complex and implications for signal recognition particle assembly [J].

Hainzl, T ;

Huang, SH ;

Sauer-Eriksson, AE .

NATURE, 2002, 417 (6890) :767-771

[8] Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle [J].

Hainzl, Tobias ;

Huang, Shenghua ;

Sauer-Eriksson, A. Elisabeth .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (38) :14911-14916

[9] Structure of the signal recognition particle interacting with the elongation-arrested ribosome [J].

Halic, M ;

Becker, T ;

Pool, MR ;

Spahn, CMT ;

Grassucci, RA ;

Frank, J ;

Beckmann, R .

NATURE, 2004, 427 (6977) :808-814

[10] Important role of the tetraloop region of 4.5S RNA in SRP binding to its receptor FtsY [J].

Jagath, JR ;

Matassova, NB ;

De Leeuw, E ;

Warnecke, JM ;

Lentzen, G ;

Rodnina, MV ;

Luirink, J ;

Wintermeyer, W .

RNA, 2001, 7 (02) :293-301