Nonenzymatic Transformation of Amorphous CaCO3 into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation

被引：35

作者：

Mueller, Werner E. G. ^{[1
]}

Neufurth, Meik ^{[1
]}

Huang, Jian ^{[2
]}

Wang, Kui ^{[2
]}

Feng, Qingling ^{[3
]}

Schroeder, Heinz C. ^{[1
]}

Diehl-Seifert, Baerbel ^{[4
]}

Munoz-Espi, Rafael ^{[5
]}

Wang, Xiaohong ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Physiol Chem, ERC Adv Investigator Grant Res Grp, D-55128 Mainz, Germany

[2] Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China

[3] Tsinghua Univ, Sch Mat Sci & Engn, Minist Educ China, Adv Mat Lab, Beijing 100084, Peoples R China

[4] NanotecMARIN GmbH, D-55128 Mainz, Germany

[5] Max Planck Inst Polymer Res, Dept Phys Chem Polymers, D-55128 Mainz, Germany

来源：

CHEMBIOCHEM | 2015年 / 16卷 / 09期

关键词：

biological activity; biomineral skeleton; bone formation; calcium phosphate; calcium; D; E peptide; ARAGONITIC CUTTLEFISH BONES; OSTEOBLAST-LIKE CELLS; CARBONIC-ANHYDRASE; EXTRACELLULAR-MATRIX; SPONGE SPICULES; SAOS-2; CELLS; VATERITE; POLYPHOSPHATE; SCAFFOLDS; WATER;

D O I：

10.1002/cbic.201500057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Studies indicate that mammalian bone formation is initiated at calcium carbonate bioseeds, a process that is driven enzymatically by carbonic anhydrase (CA). We show that amorphous calcium carbonate (ACC) and bicarbonate (HCO3-) cause induction of expression of the CA in human osteogenic SaOS-2 cells. The mineral deposits formed on the surface of the cells are rich in C, Ca and P. FTIR analysis revealed that ACC, vaterite, and aragonite, after exposure to phosphate, undergo transformation into calcium phosphate. This exchange was not seen for calcite. The changes to ACC, vaterite, and aragonite depended on the concentration of phosphate. The rate of incorporation of phosphate into ACC, vaterite, and aragonite, is significantly accelerated in the presence of a peptide rich in aspartic acid and glutamic acid. We propose that the initial CaCO3 bioseed formation is driven by CA, and that the subsequent conversion to calcium phosphate/calcium hydroxyapatite (exchange of carbonate by phosphate) is a non-enzymatic exchange process.

引用

页码：1323 / 1332

页数：10

共 81 条

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