Metabolomic analysis of the occurrence of bitter fruits on grafted oriental melon plants

被引：18
作者：
Zhang, Shuangshuang [1 ,2 ]
Nie, Lanchun [1 ,2 ,3 ]
Zhao, Wensheng [1 ,2 ,3 ]
Cui, Qiang [1 ,3 ]
Wang, Jiahao [1 ,3 ]
Duan, Yaqian [1 ,3 ]
Ge, Chang [1 ,2 ]
机构：
[1] Hebei Agr Univ, Coll Hort, Baoding, Hebei, Peoples R China
[2] Hebei Key Lab Vegetable Germplasm Innovat & Utili, Baoding, Hebei, Peoples R China
[3] Collaborat Innovat Ctr Vegetable Ind Hebei Prov, Baoding, Hebei, Peoples R China
来源：
PLOS ONE | 2019年 / 14卷 / 10期
关键词：
CUCUMIS-SATIVUS L; PHOSPHOLIPASE-D; TOTAL NITROGEN; ACID; ACCUMULATION; MECHANISM; SALINITY; PROTEIN; STRESS; CELLS;
D O I：
10.1371/journal.pone.0223707
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Grafting has been widely applied to melon (Cucumis melo L.) production to alleviate obstacles of continuous cropping and control soil-borne diseases. However, grafting often leads to a decline of fruit quality. For example, sometimes bitter fruits are produced on grafted plants. However, the underlying physiological mechanism still remains unclear. This study investigated the effects of different rootstocks on the taste of fruits of the Balengcui, an oriental melon cultivar, during summer production. The results showed that all grafted plants with Cucurbita maxima Duch. rootstocks produced bitter fruits, while non-grafted plants and plants grafted onto muskmelon rootstocks produced no bitter fruits. Liquid chromatography-mass spectrometry and metabonomic analysis were performed to investigate the mechanism underlying the occurrence of bitter fruits. Metabolite comparisons of fruits from plants grafted onto Ribenxuesong rootstocks both with non-grafted plants and plants grafted onto muskmelon rootstocks showed that 17 metabolites including phospholipids, cucurbitacins and flavonoids, exhibited changes. The three Cucurbitacins, Cucurbitacin O, Cucurbitacin C, and Cucurbitacin S, increased dramatically. The 10 phospholipids PS(18:1(9Z)/18:2 (9Z,12Z)), PS(P-18:0/15:0), PA(18:1(11Z)/18:1(11Z)), PE(16:0/18:0), PS(O-16:0/17:2 (9Z,12Z)), PI(16:0/18:2(9Z,12Z)), PA(15:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PS(P-16:0/17:2 (9Z,12Z)), PS(22:0/22:1(11Z)), and PA(17:1(9Z)/0:0)) were significantly decreased, while two PA (16:0/18:2 (9Z, 12Z) and 16:0/18:1 (11Z)), two flavonoids (pelargonidin 3-(6 ''-malonylglucoside)-5-glucoside and malvidin 3-rutinoside) significantly increased in fruits of plants grafted onto Cucurbita maxima Duch. rootstocks. These metabolites were involved in the glycerophospholipid metabolic pathway, the mevalonate pathway, and the phenylpropanoid pathway. In summary, these results showed that the bitter fruits of grafted Balengcui were caused by Cucurbita maxima Duch. rootstocks. Phospholipids, cucurbitacins, and flavonoids were the key contributors for the occurrence of bitter fruits in Balengcui melon after grafting onto Cucurbita maxima Duch. rootstocks.
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页数：13
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