Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells

被引：0

作者：

Arsov, Zoran ^{[1
,2
]}

Urbancic, Iztok ^{[1
]}

Garvas, Maja ^{[1
]}

Biglino, Daniele ^{[1
,2
]}

Ljubetic, Ajasja ^{[1
]}

Koklic, Tilen ^{[1
,2
]}

Strancar, Janez ^{[1
,2
]}

机构：

[1] Jozef Stefan Inst, Dept Solid State Phys, Lab Biophys, Ljubljana 1000, Slovenia

[2] Ctr Excellence NAMASTE, Ljubljana 1000, Slovenia

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 08期

关键词：

SINGLE-MOLECULE; MICROSCOPY; LIPOSOMES; MEMBRANES;

D O I：

10.1364/BOE.2.002083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lack of better understanding of nanoparticles targeted delivery into cancer cells calls for advanced optical microscopy methodologies. Here we present a development of fluorescence microspectroscopy (spectral imaging) based on a white light spinning disk confocal microscope with emission wavelength selection by a liquid crystal tunable filter. Spectral contrasting of images was used to localize polymer nanoparticles and cell membranes labeled with fluorophores that have substantially overlapping spectra. In addition, fluorescence microspectroscopy enabled spatially-resolved detection of small but significant effects of local molecular environment on the properties of environment-sensitive fluorescent probe. The observed spectral shift suggests that the delivery of suitably composed cancerostatic alkylphospholipid nanoparticles into living cancer cells might rely on the fusion with plasma cell membrane. (C) 2011 Optical Society of America

引用

页码：2083 / 2095

页数：13

共 34 条

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