Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins

被引：120

作者：

Kumar, Suresh ^{[1
,2
]}

Jain, Ashish ^{[3
,4
]}

Farzam, Farzin ^{[5
]}

Jia, Jingyue ^{[1
,2
]}

Gu, Yuexi ^{[1
,2
]}

Choi, Seong Won ^{[1
,2
]}

Mudd, Michal H. ^{[1
,2
]}

Claude-Taupin, Aurore ^{[1
,2
]}

Wester, Michael J. ^{[6
]}

Lidke, Keith A. ^{[5
]}

Rusten, Tor-Erik ^{[3
,4
]}

Deretic, Vojo ^{[1
,2
]}

机构：

[1] Univ New Mexico, Hlth Sci Ctr, Autophagy Inflammat & Metab Ctr Biomed Res Excell, Albuquerque, NM 87131 USA

[2] Univ New Mexico, Dept Mol Genet & Microbiol, Hlth Sci Ctr, Albuquerque, NM 87131 USA

[3] Univ Oslo, Ctr Canc Biomed, Dept Mol Cell Biol, Oslo, Norway

[4] Norwegian Radium Hosp, Inst Canc Res, Oslo, Norway

[5] Univ New Mexico, Dept Phys & Astron, Albuquerque, NM 87131 USA

[6] Univ New Mexico, Dept Math & Stat, Albuquerque, NM 87131 USA

来源：

JOURNAL OF CELL BIOLOGY | 2018年 / 217卷 / 03期

基金：

美国国家卫生研究院;

关键词：

SNARE SYNTAXIN 17; SELECTIVE AUTOPHAGY; LYSOSOME FUSION; MEMBRANE-FUSION; HOPS COMPLEX; MYCOBACTERIUM-TUBERCULOSIS; MITOCHONDRIAL DIVISION; TETHERING COMPLEXES; ENDOCYTIC PATHWAY; CONTACT SITES;

D O I：

10.1083/jcb.201708039

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Autophagy is a conserved eukaryotic process with metabolic, immune, and general homeostatic functions in mammalian cells. Mammalian autophagosomes fuse with lysosomes in a SNA RE-driven process that includes syntaxin 17 (Stx17). How Stx17 translocates to autophagosomes is unknown. In this study, we show that the mechanism of Stx17 recruitment to autophagosomes in human cells entails the small guanosine triphosphatase IRGM. Stx17 directly interacts with IRGM, and efficient Stx17 recruitment to autophagosomes requires IRGM. Both IRGM and Stx17 directly interact with mammalian Atg8 proteins, thus being guided to autophagosomes. We also show that Stx17 is significant in defense against infectious agents and that Stx17-IRGM interaction is targeted by an HIV virulence factor Nef.

引用

页码：997 / 1013

页数：17

共 23 条

[1] Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens
Suresh Kumar
Ashish Jain
Seong Won Choi
Gustavo Peixoto Duarte da Silva
Lee Allers
Michal H. Mudd
Ryan Scott Peters
Jan Haug Anonsen
Tor-Erik Rusten
Michael Lazarou
Vojo Deretic
Nature Cell Biology, 2020, 22 : 973 - 985
[2] Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens
Kumar, Suresh
Jain, Ashish
Choi, Seong Won
Duarte da Silva, Gustavo Peixoto
Allers, Lee
Mudd, Michal H.
Peters, Ryan Scott
Anonsen, Jan Haug
Rusten, Tor-Erik
Lazarou, Michael
Deretic, Vojo
NATURE CELL BIOLOGY, 2020, 22 (08) : 973 - +
[3] Molecular mechanisms of autophagy in plants: Role of ATG8 proteins in formation and functioning of autophagosomes
Ryabovol, V. V.
Minibayeva, F. V.
BIOCHEMISTRY-MOSCOW, 2016, 81 (04) : 348 - 363
[4] Molecular mechanisms of autophagy in plants: Role of ATG8 proteins in formation and functioning of autophagosomes
V. V. Ryabovol
F. V. Minibayeva
Biochemistry (Moscow), 2016, 81 : 348 - 363
[5] Author Correction: Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens
Suresh Kumar
Ashish Jain
Seong Won Choi
Gustavo Peixoto Duarte da Silva
Lee Allers
Michal H. Mudd
Ryan Scott Peters
Jan Haug Anonsen
Tor-Erik Rusten
Michael Lazarou
Vojo Deretic
Nature Cell Biology, 2020, 22 : 1286 - 1286
[6] Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs
Gu, Yuexi
Abudu, Yakubu Princely
Kumar, Suresh
Bissa, Bhawana
Choi, Seong Won
Jia, Jingyue
Lazarou, Michael
Eskelinen, Eeva-Liisa
Johansen, Terje
Deretic, Vojo
EMBO JOURNAL, 2019, 38 (22):
[7] Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs
Javed, Ruheena
Jain, Ashish
Duque, Thabata
Hendrix, Emily
Paddar, Masroor Ahmad
Khan, Sajjad
Claude-Taupin, Aurore
Jia, Jingyue
Allers, Lee
Wang, Fulong
Mudd, Michal
Timmins, Graham
Lidke, Keith
Rusten, Tor Erik
Akepati, Prithvi Reddy
He, Yi
Reggiori, Fulvio
Eskelinen, Eeva-Liisa
Deretic, Vojo
EMBO JOURNAL, 2023, 42 (14):
[8] Development of new tools to study membrane-anchored mammalian Atg8 proteins
Park, Sang-Won
Jeon, Pureum
Yamasaki, Akinori
Lee, Hye Eun
Choi, Haneul
Mun, Ji Young
Jun, Yong-Woo
Park, Ju-Hui
Lee, Seung-Hwan
Lee, Soo-Kyeong
Lee, You-Kyung
Song, Hyun Kyu
Lazarou, Michael
Cho, Dong-Hyong
Komatsu, Masaaki
Noda, Nobuo N.
Jang, Deok-Jin
Lee, Jin-A
AUTOPHAGY, 2023, 19 (05) : 1424 - 1443
[9] Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens (vol 22, pg 973, 2020)
Kumar, Suresh
Jain, Ashish
Choi, Seong Won
da Silva, Gustavo Peixoto Duarte
Allers, Lee
Mudd, Michal H.
Peters, Ryan Scott
Anonsen, Jan Haug
Rusten, Tor-Erik
Lazarou, Michael
Deretic, Vojo
NATURE CELL BIOLOGY, 2020, 22 (10) : 1286 - 1286
[10] STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism
Xian, Hongxu
Yang, Qiaoyun
Liou, Yih-Cherng
FASEB JOURNAL, 2019, 33

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