Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins

被引：121

作者：

Kumar, Suresh ^{[1
,2
]}

Jain, Ashish ^{[3
,4
]}

Farzam, Farzin ^{[5
]}

Jia, Jingyue ^{[1
,2
]}

Gu, Yuexi ^{[1
,2
]}

Choi, Seong Won ^{[1
,2
]}

Mudd, Michal H. ^{[1
,2
]}

Claude-Taupin, Aurore ^{[1
,2
]}

Wester, Michael J. ^{[6
]}

Lidke, Keith A. ^{[5
]}

Rusten, Tor-Erik ^{[3
,4
]}

Deretic, Vojo ^{[1
,2
]}

机构：

[1] Univ New Mexico, Hlth Sci Ctr, Autophagy Inflammat & Metab Ctr Biomed Res Excell, Albuquerque, NM 87131 USA

[2] Univ New Mexico, Dept Mol Genet & Microbiol, Hlth Sci Ctr, Albuquerque, NM 87131 USA

[3] Univ Oslo, Ctr Canc Biomed, Dept Mol Cell Biol, Oslo, Norway

[4] Norwegian Radium Hosp, Inst Canc Res, Oslo, Norway

[5] Univ New Mexico, Dept Phys & Astron, Albuquerque, NM 87131 USA

[6] Univ New Mexico, Dept Math & Stat, Albuquerque, NM 87131 USA

来源：

JOURNAL OF CELL BIOLOGY | 2018年 / 217卷 / 03期

基金：

美国国家卫生研究院;

关键词：

SNARE SYNTAXIN 17; SELECTIVE AUTOPHAGY; LYSOSOME FUSION; MEMBRANE-FUSION; HOPS COMPLEX; MYCOBACTERIUM-TUBERCULOSIS; MITOCHONDRIAL DIVISION; TETHERING COMPLEXES; ENDOCYTIC PATHWAY; CONTACT SITES;

D O I：

10.1083/jcb.201708039

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Autophagy is a conserved eukaryotic process with metabolic, immune, and general homeostatic functions in mammalian cells. Mammalian autophagosomes fuse with lysosomes in a SNA RE-driven process that includes syntaxin 17 (Stx17). How Stx17 translocates to autophagosomes is unknown. In this study, we show that the mechanism of Stx17 recruitment to autophagosomes in human cells entails the small guanosine triphosphatase IRGM. Stx17 directly interacts with IRGM, and efficient Stx17 recruitment to autophagosomes requires IRGM. Both IRGM and Stx17 directly interact with mammalian Atg8 proteins, thus being guided to autophagosomes. We also show that Stx17 is significant in defense against infectious agents and that Stx17-IRGM interaction is targeted by an HIV virulence factor Nef.

引用

页码：997 / 1013

页数：17

共 72 条

[1] ATG8 Family Proteins Act as Scaffolds for Assembly of the ULK Complex SEQUENCE REQUIREMENTS FOR LC3-INTERACTING REGION (LIR) MOTIFS [J].

Alemu, Endalkachew Ashenafi ;

Lamark, Trond ;

Torgersen, Knut Martin ;

Birgisdottir, Aasa Birna ;

Larsen, Kenneth Bowitz ;

Jain, Ashish ;

Olsvik, Hallvard ;

Overvatn, Aud ;

Kirkin, Vladimir ;

Johansen, Terje .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (47) :39275-39290

[2] A Role for the Ancient SNARE Syntaxin 17 in Regulating Mitochondrial Division [J].

Arasaki, Kohei ;

Shimizu, Hiroaki ;

Mogari, Hirofumi ;

Nishida, Naoki ;

Hirota, Naohiko ;

Furuno, Akiko ;

Kudo, Yoshihisa ;

Baba, Misuzu ;

Baba, Norio ;

Cheng, Jinglei ;

Fujimoto, Toyoshi ;

Ishihara, Naotada ;

Ortiz-Sandoval, Carolina ;

Barlow, Lael D. ;

Raturi, Arun ;

Dohmae, Naoshi ;

Wakana, Yuichi ;

Inoue, Hiroki ;

Tani, Katsuko ;

Dacks, Joel B. ;

Simmen, Thomas ;

Tagaya, Mitsuo .

DEVELOPMENTAL CELL, 2015, 32 (03) :304-317

[3] MEMBRANE FUSION A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly [J].

Baker, Richard W. ;

Jeffrey, Philip D. ;

Zick, Michael ;

Phillips, Ben P. ;

Wickner, William T. ;

Hughson, Frederick M. .

SCIENCE, 2015, 349 (6252) :1111-1114

[4] CORVET and HOPS tethering complexes - coordinators of endosome and lysosome fusion [J].

Balderhaar, Henning J. Kleine ;

Ungermann, Christian .

JOURNAL OF CELL SCIENCE, 2013, 126 (06) :1307-1316

[5] Network organization of the human autophagy system [J].

Behrends, Christian ;

Sowa, Mathew E. ;

Gygi, Steven P. ;

Harper, J. Wade .

NATURE, 2010, 466 (7302) :68-U84

[6] The LIR motif - crucial for selective autophagy [J].

Birgisdottir, Asa Birna ;

Lamark, Trond ;

Johansen, Terje .

JOURNAL OF CELL SCIENCE, 2013, 126 (15) :3237-3247

[7] Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls [J].

Burton, Paul R. ;

Clayton, David G. ;

Cardon, Lon R. ;

Craddock, Nick ;

Deloukas, Panos ;

Duncanson, Audrey ;

Kwiatkowski, Dominic P. ;

McCarthy, Mark I. ;

Ouwehand, Willem H. ;

Samani, Nilesh J. ;

Todd, John A. ;

Donnelly, Peter ;

Barrett, Jeffrey C. ;

Davison, Dan ;

Easton, Doug ;

Evans, David ;

Leung, Hin-Tak ;

Marchini, Jonathan L. ;

Morris, Andrew P. ;

Spencer, Chris C. A. ;

Tobin, Martin D. ;

Attwood, Antony P. ;

Boorman, James P. ;

Cant, Barbara ;

Everson, Ursula ;

Hussey, Judith M. ;

Jolley, Jennifer D. ;

Knight, Alexandra S. ;

Koch, Kerstin ;

Meech, Elizabeth ;

Nutland, Sarah ;

Prowse, Christopher V. ;

Stevens, Helen E. ;

Taylor, Niall C. ;

Walters, Graham R. ;

Walker, Neil M. ;

Watkins, Nicholas A. ;

Winzer, Thilo ;

Jones, Richard W. ;

McArdle, Wendy L. ;

Ring, Susan M. ;

Strachan, David P. ;

Pembrey, Marcus ;

Breen, Gerome ;

St Clair, David ;

Caesar, Sian ;

Gordon-Smith, Katherine ;

Jones, Lisa ;

Fraser, Christine ;

Green, Elain K. .

NATURE, 2007, 447 (7145) :661-678

[8] TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis [J].

Chauhan, Santosh ;

Kumar, Suresh ;

Jain, Ashish ;

Ponpuak, Marisa ;

Mudd, Michal H. ;

Kimura, Tomonori ;

Choi, Seong Won ;

Peters, Ryan ;

Mandell, Michael ;

Bruun, Jack-Ansgar ;

Johansen, Terje ;

Deretic, Vojo .

DEVELOPMENTAL CELL, 2016, 39 (01) :13-27

[9] IRGM Governs the Core Autophagy Machinery to Conduct Antimicrobial Defense [J].

Chauhan, Santosh ;

Mandell, Michael A. ;

Deretic, Vojo .

MOLECULAR CELL, 2015, 58 (03) :507-521

[10] Looking for natural patterns in data - Part 1. Density-based approach [J].

Daszykowski, M ;

Walczak, B ;

Massart, DL .

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 2001, 56 (02) :83-92

← 1 2 3 4 5 6 7 8 →