Segmental Isotopic Labeling of Ubiquitin Chains To Unravel Monomer-Specific Molecular Behavior

被引：24

作者：

Castaneda, Carlos A. ^{[3
]}

Spasser, Liat ^{[1
,2
]}

Bavikar, Sudhir N. ^{[1
,2
]}

Brik, Ashraf ^{[1
,2
]}

Fushman, David ^{[3
]}

机构：

[1] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel

[3] Univ Maryland, Dept Chem & Biochem, Ctr Biomol Struct & Org, College Pk, MD 20742 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 47期

基金：

美国国家科学基金会; 以色列科学基金会;

关键词：

chain structures; isotopic labeling; protein structures; semisynthesis; ubiquitin; TOTAL CHEMICAL-SYNTHESIS; POLYUBIQUITIN CHAINS; INTERDOMAIN MOBILITY; DOMAINS; BINDING; TETRAUBIQUITIN; PROTEASOME; LIGATION; MODEL;

D O I：

10.1002/anie.201104649

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

"Seeing" each monomer in ubiquitin chains: By combination of the chemical synthesis of one ubiquitin monomer with recombinant expression of its 15N-containing counterpart, the labeling of ubiquitins in non-enzymatically assembled di-ubiquitin chains was achieved, to enable monomer-specific studies in solution. This allowed the first characterization of the structure, dynamics, intrachain interactions, and ligand binding of Lys33-linked di-ubiquitin. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：11210 / 11214

页数：5

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