Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole

被引:10
作者
Guedes, Thays de Andrade [1 ]
Moreira-de-Sousa, Cristina [1 ]
Lima, Hellen Maria Soares [1 ]
Grella, Tatiane Caroline [1 ]
Socolowski, Priscila Cintra [1 ]
Fontanetti, Carmem Silvia [1 ]
机构
[1] UNESP Sao Paulo State Univ, Dept Biol, IB Inst Biociencias, Ave 24-A,1515, BR-13506900 Rio Claro, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Stress protein; TUNEL; phenylpyrazole; Tilapia; immunostaining; ZEBRAFISH DANIO-RERIO; IN-SITU; TOXICITY EVALUATION; SUGARCANE VINASSE; CELL-DEATH; LIVER; RESPONSES; HISTOPATHOLOGY; SUSCEPTIBILITY; FORMULATIONS;
D O I
10.1080/03601234.2020.1766898
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent (R) WS 800 and Curbix (R) SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of Oreochromis niloticus. The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals. Nevertheless, variations in HSP70 and DNA fragmentation levels, endpoint of cell repair system response and cellular death, respectively, were observed in several groups. These results indicate that the cell repair machinery was efficient when in contact with residual concentrations of insecticides. However, the DNA fragmentation detected by the TUNEL method suggests that even in face of the cytoprotective action of the HSP70 protein, there are damages that become irreparable. To finish, it is worth mentioning that given the results obtained from residual concentrations, use in the field should be with caution.
引用
收藏
页码:687 / 693
页数:7
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