Identification and characterization of long non-coding RNA in prenatal and postnatal skeletal muscle of sheep

被引：16

作者：

Li, Cun-Yuan ^{[1
]}

Li, Xiaoyue ^{[1
]}

Liu, Zhijin ^{[1
]}

Ni, Wei ^{[1
]}

Zhang, Xiangyu ^{[1
]}

Hazi, Wureli ^{[2
]}

Ma, Qiman ^{[2
]}

Zhang, Yunfeng ^{[2
]}

Cao, Yang ^{[1
]}

Qi, Jiangjiao ^{[1
]}

Yao, Yang ^{[1
]}

Feng, Lin ^{[1
]}

Wang, Dawei ^{[1
]}

Hou, Xiaoxu ^{[1
]}

Yu, Shuting ^{[1
]}

Liu, Li ^{[1
]}

Zhang, Mengdan ^{[1
]}

Hu, Shengwei ^{[1
]}

机构：

[1] Shihezi Univ, Coll Life Sci, Shihezi 832003, Xinjiang, Peoples R China

[2] Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

来源：

GENOMICS | 2019年 / 111卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Sheep; Long non-coding RNAs; Skeletal muscle; Expression profiles; Functional prediction; GENE; PATHWAY; DIFFERENTIATION; EXPRESSION; TRANSCRIPTION; LANDSCAPE; REGULATOR; REVEALS; SPECIFICATION; HYPERTROPHY;

D O I：

10.1016/j.ygeno.2018.01.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

lncRNAs are a class of transcriptional RNA molecules of > 200 nucleotides in length. However, the overall expression pattern and function of lncRNAs in sheep muscle is not clear. Here, we identified 1566 lncRNAs and 404 differentially expressed lncRNAs in sheep muscle from prenatal (110 days of fetus) and postnatal (2 to 3 years old of adult sheep) developmental stages by using RNA-seq technology. Several lncRNAs were identified by using RT-PCR and DNA sequencing. The expression levels of several lncRNAs were confirmed by qRT-PCR. We analyzed the effect of lncRNAs that act cis to the target genes. lncRNA targeting genes were involved in signaling pathways associated with growth and development of muscle by GO and KEGG enrichment analysis. Through our study, we provide a comprehensive expression profile of muscle lncRNAs in sheep, which provides valuable resources for further understanding genetic regulation of muscle growth and development from the perspective of lncRNA.

引用

页码：133 / 141

页数：9