Affordable pediatric CD4 counting by flow cytometry in Malawi

被引:4
作者
MacLennan, Calman A. [1 ,2 ,3 ,4 ,5 ]
Dzumani, Felix [6 ]
Namarika, Alinane [6 ]
Moons, Peter [7 ]
Senga, Edward [6 ]
Molyneux, Makohn E. [3 ]
Drayson, Mark T. [1 ,2 ]
Bunn, James E. G. [7 ,8 ]
机构
[1] Univ Birmingham, Inst Biomed Res, Med Res Council Ctr Immune Regulat, Birmingham B15 2TT, W Midlands, England
[2] Univ Birmingham, Inst Biomed Res, Div Immun & Infect, Clin Immunol Serv, Birmingham B15 2TT, W Midlands, England
[3] Univ Malawi, Coll Med, Malawi Liverpool Wellcome Trust Clin Res Programm, Blantyre, Malawi
[4] Univ Liverpool, Sch Infect & Host Def, Div Med Microbiol, Liverpool L69 3BX, Merseyside, England
[5] Univ Malawi, Coll Med, Dept Microbiol, Blantyre, Malawi
[6] Univ Malawi, Coll Med, Dept Biochem, Blantyre, Malawi
[7] Univ Malawi, Coll Med, Dept Pediat, Blantyre, Malawi
[8] Univ Malawi, Coll Med, Dept Community Hlth, Blantyre, Malawi
基金
英国惠康基金;
关键词
CD4 lymphocyte count; flow cytometry; Malawi; HIV; pediatrics;
D O I
10.1002/cyto.b.20411
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Rapid expansion of antiretroviral therapy in Malawi has occurred in the relative absence of suitable pediatric CD4 counting facilities. We have recently validated in adults a simplified affordable flow cytometric CD4 counting method, the Blantyre count. There is a need for this technology to transfer to government laboratories run by local staff, and to be validated in children, where %CD4/lymphocyte values are required. Methods: We assessed agreement of %CD4/lymphocyte values determined by the Blantyre count and Panleucogate methods on an EPICS XL-MCL flow cytometer on 113 venous blood samples from HIV-seropositive children in Blantyre, Malawi. All assays were performed by two Malawian laboratory technicians. Results: Overall bias between the two methods was -0.13% (95% CI -0.37 to 0.11) and limits of agreement were -2.69 to 2.43% (95% CI -3.11 to -2.27 and 2.01 to 2.85). Limits of agreement were within -3.00 and 3.00 for each laboratory technician. Coefficient of variation for the Blantyre count assay was 2.0% and samples showed good stability over 5 days. Conclusions: The Blantyre count method can accurately determine %CD4/lymphocyte values in blood of HIV-seropositive children on an EPIC XL-MCL flow cytometer at a reagent cost of US $0.21 per test or less. The assay can be competently carried out by local laboratory technicians. (C) 2008 Clinical Cytometry Society.
引用
收藏
页码:S90 / S97
页数:8
相关论文
共 50 条
[41]   Optically induced flow cytometry for continuous microparticle counting and sorting [J].
Lin, Yen-Heng ;
Lee, Gwo-Bin .
BIOSENSORS & BIOELECTRONICS, 2008, 24 (04) :572-578
[42]   Laser rastering flow cytometry: fast cell counting and identification [J].
Vacca, G. ;
Junnarkar, M. R. ;
Goldblatt, N. R. ;
Yee, M. W. ;
Van Slyke, B. M. ;
Briese, T. C. .
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VII, 2009, 7182
[43]   PREDICTION OF CD4 COUNT FROM CD4 PERCENTAGE - EXPERIENCE FROM 3 LABORATORIES [J].
KIDD, PG ;
CHENG, SC ;
PAXTON, H ;
LANDAY, A ;
GELMAN, R .
AIDS, 1993, 7 (07) :933-940
[44]   Absolute Counting of Neutrophils in Whole Blood Using Flow Cytometry [J].
Brunck, Marion E. G. ;
Andersen, Stacey B. ;
Timmins, Nicholas E. ;
Osborne, Geoffrey W. ;
Nielsen, Lars K. .
CYTOMETRY PART A, 2014, 85A (12) :1057-1064
[45]   Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry [J].
Saraiva, Luisa ;
Wang, Lili ;
Kammel, Martin ;
Kummrow, Andreas ;
Atkinson, Eleanor ;
Lee, Ji Youn ;
Yalcinkaya, Burhanettin ;
Akgoez, Muslum ;
Hoeckner, Jana ;
Ruf, Andreas ;
Engel, Andrea ;
Zhang, Yu-Zhong ;
O'Shea, Orla ;
Sassi, Maria Paola ;
Divieto, Carla ;
Lekishvili, Tamara ;
Campbell, Jonathan ;
Liu, Yingying ;
Wang, Jing ;
Stebbings, Richard ;
Gaigalas, Adolfas K. ;
Rigsby, Peter ;
Neukammer, Joerg ;
Vessillier, Sandrine .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2019, 96 (06) :508-513
[46]   CD4 T cell measurements in the management of antiretroviral therapy - A review with an emphasis on pediatric HIV-infected patients [J].
O'Gorman, Maurice R. G. ;
Zijenah, Lynn S. .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2008, 74B :S19-S26
[47]   DETECTION OF APOPTOSIS OF IMMATURE CD4+8+ THYMOCYTES BY FLOW-CYTOMETRY [J].
SWAT, W ;
IGNATOWICZ, L ;
KISIELOW, P .
JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 137 (01) :79-87
[48]   New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-Infected pediatric patients [J].
Pattanapanyasat, Kovit ;
Sukapirom, Kasama ;
Kowawisatsut, Laddawan ;
Thepthai, Charin .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2008, 74B :S98-S106
[49]   CD4 antigen loss in bronchoalveolar lavage fluid with neutrophilia and infectious organisms: A diagnostic pitfall when analyzing T-cell subsets by flow cytometry [J].
Qiu, Lianqun ;
Love, Jason E. ;
Simonson, William ;
Fromm, Jonathan R. ;
Cherian, Sindhu .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2025,
[50]   Administration of interleukin-7 increases CD4 T cells in idiopathic CD4 lymphocytopenia [J].
Sheikh, Virginia ;
Porter, Brian O. ;
DerSimonian, Rebecca ;
Kovacs, Stephen B. ;
Thompson, William L. ;
Perez-Diez, Ainhoa ;
Freeman, Alexandra F. ;
Roby, Gregg ;
Mican, JoAnn ;
Pau, Alice ;
Rupert, Adam ;
Adelsberger, Joseph ;
Higgins, Jeanette ;
Bourgeois, Jeffrey S., Jr. ;
Jensen, Stig M. R. ;
Morcock, David R. ;
Burbelo, Peter D. ;
Osnos, Leah ;
Maric, Irina ;
Natarajan, Ven ;
Croughs, Therese ;
Yao, Michael D. ;
Estes, Jacob D. ;
Sereti, Irini .
BLOOD, 2016, 127 (08) :977-988