Peptide affinity reagents for AAV capsid recognition and purification

被引：13

作者：

Pulicherla, N. ^{[1
]}

Asokan, A. ^{[1
,2
,3
]}

机构：

[1] Univ N Carolina, Gene Therapy Ctr, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Mol & Cellular Biophys Program, Chapel Hill, NC 27599 USA

来源：

GENE THERAPY | 2011年 / 18卷 / 10期

关键词：

AAV vectors; phage display; affinity column chromatography; RECOMBINANT ADENOASSOCIATED VIRUS; VIRAL VECTOR PRODUCTION; SCALE; CHROMATOGRAPHY; SEROTYPES; DISPLAY; CELLS; TITER;

D O I：

10.1038/gt.2011.46

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors. Gene Therapy (2011) 18, 1020-1024; doi:10.1038/gt.2011.46; published online 14 April 2011

引用

页码：1020 / 1024

页数：5

共 20 条

[1] Metabolic biotinylation provides a unique platform for the purification and targeting of multiple AAV vector serotypes [J].

Arnold, Gregory S. ;

Sasser, A. Kate ;

Stachler, Matthew D. ;

Bartlett, Jeffrey S. .

MOLECULAR THERAPY, 2006, 14 (01) :97-106

[2] Critical assessment of current adeno-associated viral vector production and quantification methods [J].

Aucoin, Marc G. ;

Perrier, Michel ;

Kamen, Amine A. .

BIOTECHNOLOGY ADVANCES, 2008, 26 (01) :73-88

[3] Large-Scale Adeno-Associated Viral Vector Production Using a Herpesvirus-Based System Enables Manufacturing for Clinical Studies [J].

Clement, Nathalie ;

Knop, David R. ;

Byrne, Barry J. .

HUMAN GENE THERAPY, 2009, 20 (08) :796-806

[4] Production and characterization of adeno-associated viral vectors [J].

Grieger, Joshua C. ;

Choi, Vivian W. ;

Samulski, R. Jude .

NATURE PROTOCOLS, 2006, 1 (03) :1412-1428

[5] Engineering adeno-associated virus for one-step purification via immobilized metal affinity chromatography [J].

Koerber, James T. ;

Jang, Jae-Hyung ;

Yu, Julie H. ;

Kane, Ravi S. ;

Schaffer, David V. .

HUMAN GENE THERAPY, 2007, 18 (04) :367-378

[6] Rapid, Simple, and Versatile Manufacturing of Recombinant Adeno-Associated Viral Vectors at Scale [J].

Lock, Martin ;

Alvira, Mauricio ;

Vandenberghe, Luk H. ;

Samanta, Arabinda ;

Toelen, Jaan ;

Debyser, Zeger ;

Wilson, James M. .

HUMAN GENE THERAPY, 2010, 21 (10) :1259-1271

[7]

Mitchell AM, 2010, CURR GENE THER, V10, P319

[8]

Petrenko VA, 2008, EXPERT OPIN DRUG DEL, V5, P825, DOI [10.1517/17425247.5.8.825, 10.1517/17425247.5.8.825 ]

[9] Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity [J].

Rabinowitz, JE ;

Rolling, F ;

Li, CW ;

Conrath, H ;

Xiao, WD ;

Xiao, X ;

Samulski, RJ .

JOURNAL OF VIROLOGY, 2002, 76 (02) :791-801

[10] Display technologies: Application for the discovery of drug and gene delivery agents [J].

Sergeeva, Anna ;

Kolonin, Mikhail G. ;

Molldrem, Jeffrey J. ;

Pasqualini, Renata ;

Arap, Wadih .

ADVANCED DRUG DELIVERY REVIEWS, 2006, 58 (15) :1622-1654

← 1 2 →