Improved pulsed-field gel electrophoresis procedure for the analysis of Flavobacterium columnare isolates previously affected by DNA degradation

被引：5
作者：
Soto, Esteban [1 ,2 ]
Mauel, Michael [1 ,2 ]
Lawrence, Mark [2 ]
机构：
[1] Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA
[2] Mississippi State Univ, Coll Vet Med, Mississippi State, MS 39762 USA
来源：
VETERINARY MICROBIOLOGY | 2008年 / 128卷 / 1-2期
关键词：
Flavobacterium columnare; PFGE; formaldehyde fixation; DNA degradation;
D O I：
10.1016/j.vetmic.2007.10.001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Flavobacterium columnare is a fresh water bacterium that causes columnaris diseases in over 36 fish species. Intra-species typing of F columnare can be performed by pulsed-field gel electrophoresis (PFGE). However, this method is hampered by the degradation of chromosomal DNA in about 10% of strains. In the current study, DNA degradation problems caused by extracellular DNases were overcome by fixation of cells with formaldehyde prior to isolation. The results substantiate that after problems due to DNases are overcome, PFGE analysis is a reproducible highly discriminating epidemiological method for studying F columnare isolates regardless of fish host. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：207 / 212
页数：6
相关论文
共 50 条
  • [1] Optimization of Pulsed-field Gel Electrophoresis Procedure for Bacillus cereus
    ZHANG Hui Juan
    PAN Zhuo
    WEI Jian Chun
    ZHANG En Min
    CAI Hong
    LIANG Xu Dong
    LI Wei
    Biomedical and Environmental Sciences, 2016, 29 (03) : 233 - 237
  • [2] A new method of DNA preparation for pulsed-field gel electrophoresis analysis
    Fiett, J
    Kucharezyk, K
    Gniadkowski, M
    JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (03) : 433 - 436
  • [3] Application of pulsed-field gel electrophoresis for the discrimination of leptospiral isolates in Brazil
    Romero, E. C.
    Blanco, R. M.
    Galloway, R. L.
    LETTERS IN APPLIED MICROBIOLOGY, 2009, 48 (05) : 623 - 627
  • [4] Pulsed-field gel electrophoresis of Campylobacter jejuni sheep abortion isolates
    Mannering, SA
    West, DM
    Fenwick, SG
    Marchant, RM
    O'Connell, K
    VETERINARY MICROBIOLOGY, 2006, 115 (1-3) : 237 - 242
  • [5] Subtyping of Salmonella isolates on retail raw chicken in China by pulsed-field gel electrophoresis and plasmid analysis
    Wang, Yin
    Yang, Baowei
    Cui, Yue
    Alali, Walid Q.
    Xia, Xiaodong
    Xi, Meili
    Wang, Xin
    Shi, Xianming
    Wang, Dapeng
    Meng, Jianghong
    FOOD CONTROL, 2015, 47 : 420 - 426
  • [6] Customizable BAC-based DNA markers for pulsed-field gel electrophoresis
    Wong, Yin Cheng
    Ng, Allan Wee Ren
    Osahor, Andrew
    Narayanan, Kumaran
    ANALYTICAL BIOCHEMISTRY, 2024, 693
  • [7] Modified pulsed-field gel electrophoresis method for DNA degradation-sensitive Salmonella enterica and Escherichia coli strains
    Liesegang, A
    Tschäpe, H
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 291 (08) : 645 - 648
  • [8] Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis
    Chou, Hsiao-Hui
    Chiou, Chien-Shun
    Chen, Kuo-Wei
    Li, Shu-Ying
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 57 (04) : 451 - 454
  • [9] Antimicrobial resistance and genetic analysis of multi-drug resistant Klebsiella pneumoniae isolates by pulsed-field gel electrophoresis
    Pour, Marya Teimour
    Gheysarzadeh, Ali
    Pakzad, Iraj
    Valadbeigi, Hassan
    Maleki, Abbas
    Sadeghifard, Nourkhoda
    GENE REPORTS, 2020, 19
  • [10] Comparison of pulsed-field gel electrophoresis with random amplified polymorphic DNA for typing of Mycoplasma synoviae
    Marois, C
    Dufour-Gesbert, F
    Kempf, I
    VETERINARY MICROBIOLOGY, 2001, 79 (01) : 1 - 9
12345