C-elegans telomeres contain G-strand and C-strand overhangs that are bound by distinct proteins

被引：100

作者：

Raices, Marcela ^{[1
]}

Verdun, Ramiro E. ^{[1
]}

Compton, Sarah A. ^{[2
]}

Haggblom, Candy I. ^{[1
]}

Griffith, Jack D. ^{[2
]}

Dillin, Andrew ^{[1
]}

Karlseder, Jan ^{[1
]}

机构：

[1] Salk Inst Biol Studies, La Jolla, CA 92037 USA

[2] Univ N Carolina, Lineberger Comprehens Canc Ctr, Sch Med, Chapel Hill, NC 27599 USA

来源：

CELL | 2008年 / 132卷 / 05期

关键词：

D O I：

10.1016/j.cell.2007.12.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-strand extensions of the G strand of telomeres are known to be critical for chromosome-end protection and length regulation. Here, we report that in C. elegans, chromosome termini possess 30 G-strand overhangs as well as 50 C-strand overhangs. C tails are as abundant as G tails and are generated by a well-regulated process. These two classes of overhangs are bound by two single-stranded DNA binding proteins, CeOB1 and CeOB2, which exhibit specificity for G-rich or C-rich telomeric DNA. Strains of worms deleted for CeOB1 have elongated telomeres as well as extended G tails, whereas CeOB2 deficiency leads to telomere-length heterogeneity. Both CeOB1 and CeOB2 contain OB (oligo-saccharide/oligo-nucleotide binding) folds, which exhibit structural similarity to the second and first OB folds of the mammalian telomere binding protein hPOT1, respectively. Our results suggest that C. elegans telomere homeostasis relies on a novel mechanism that involves 50 and 30 single-stranded termini.

引用

页码：745 / 757

页数：13

共 53 条

[51] SACCHAROMYCES TELOMERES ACQUIRE SINGLE-STRAND TG(1-3) TAILS LATE IN S-PHASE [J].