Histomorphometric, physical, and mechanical effects of spaceflight and insulin-like growth factor-I on rat long bones

被引:39
作者
Bateman, TA
Zimmerman, RJ
Ayers, RA
Ferguson, VL
Chapes, SK
Simske, SJ
机构
[1] Univ Colorado, Dept Aerosp Engn Sci, Boulder, CO 80309 USA
[2] Chiron Corp, Emeryville, CA 94608 USA
[3] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA
基金
美国国家航空航天局;
关键词
spaceflight; insulin-like growth factor-I; histomorphometry; mechanical testing; microhardness;
D O I
10.1016/S8756-3282(98)00135-5
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Previous experiments have shown that skeletal unloading resulting from exposure to microgravity induces osteopenia in rats. In maturing rats, this is primarily a function of reduced formation, rather than increased resorption, Insulin-like growth factor-I (IGF-I) stimulates bone formation by increasing collagen synthesis by osteoblasts. The ability of IGF-I to prevent osteopenia otherwise caused by spaceflight was investigated in 12 rats flown for 10 days aboard the Space Shuttle, STS-77, The effect IGF-I had on cortical bone metabolism was generally anabolic, For example, humerus periosteal bone formation increased a significant 37.6% for the spaceflight animals treated with IGF-I, whereas the ground controls increased 24.7%, This increase in humeral bone formation at the periosteum is a result of an increased. percent mineralizing perimeter (%Min.Pm), rather than mineral apposition rate (MAR), for both spaceflight and ground control rats. However, IGF-I did inhibit humerus endocortical bone formation in both the spaceflight and ground control rats (38.1% and 39.2%, respectively) by limiting MAR. This effect was verified in a separate ground-based study. Similar histomorphometric results for spaceflight and ground control rats suggest that IGF-I effects occur during normal weight bearing and during spaceflight, Microhardness measurements of the newly formed bone indicate that the quality of the bone formed during IGF-I treatment or spaceflight was not adversely altered. Spaceflight did not consistently change the structural (force-deflection) properties of the femur or humerus when tested in three-point bending. IGF-I significantly increased femoral maximum and fracture strength. (Bone 23:527-635; 1998) (C) 1998 by Elsevier Science Inc. All rights reserved.
引用
收藏
页码:527 / 535
页数:9
相关论文
共 52 条
[1]   THE EFFECTS OF SIMULATED WEIGHTLESSNESS ON BONE BIOMECHANICAL AND BIOCHEMICAL-PROPERTIES IN THE MATURING RAT [J].
ABRAM, AC ;
KELLER, TS ;
SPENGLER, DM .
JOURNAL OF BIOMECHANICS, 1988, 21 (09) :755-&
[2]  
Ayers R A, 1996, Biomed Sci Instrum, V32, P251
[3]   TREATMENT OF OVARIECTOMIZED RATS WITH THE COMPLEX OF RHIGF-I/IGFBP-3 INCREASES CORTICAL AND CANCELLOUS BONE MASS AND IMPROVES STRUCTURE IN THE FEMORAL-NECK [J].
BAGI, CM ;
DELEON, E ;
BROMMAGE, R ;
ROSEN, D ;
SOMMER, A .
CALCIFIED TISSUE INTERNATIONAL, 1995, 57 (01) :40-46
[4]  
BAGI CM, 1995, BONE, V16, pS263, DOI 10.1016/S8756-3282(95)80073-5
[5]  
BIKLE DD, 1994, J BONE MINER RES, V9, P1789
[6]   THE MOLECULAR RESPONSE OF BONE TO GROWTH-HORMONE DURING SKELETAL UNLOADING - REGIONAL DIFFERENCES [J].
BIKLE, DD ;
HARRIS, J ;
HALLORAN, BP ;
CURRIER, PA ;
TANNER, S ;
MOREYHOLTON, E .
ENDOCRINOLOGY, 1995, 136 (05) :2099-2109
[7]   IMPACT OF SKELETAL UNLOADING ON BONE-FORMATION - ROLE OF SYSTEMIC AND LOCAL FACTORS [J].
BIKLE, DD ;
HALLORAN, BP ;
MOREYHOLTON, E .
ACTA ASTRONAUTICA, 1994, 33 :119-129
[8]   ALTERED SKELETAL PATTERN OF GENE-EXPRESSION IN RESPONSE TO SPACEFLIGHT AND HINDLIMB ELEVATION [J].
BIKLE, DD ;
HARRIS, J ;
HALLORAN, BP ;
MOREYHOLTON, E .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1994, 267 (06) :E822-E827
[9]   EFFECTS OF REHYDRATION STATE ON THE FLEXURAL PROPERTIES OF WHOLE MOUSE LONG BONES [J].
BROZ, JJ ;
SIMSKE, SJ ;
GREENBERG, AR ;
LUTTGES, MW .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1993, 115 (04) :447-449