Determination of perfluorocarboxylic acids by gas-liquid chromatography in rat tissues

被引：27
|
作者：
Kudo, N [1 ]
Bandai, N [1 ]
Kawashima, Y [1 ]
机构：
[1] Josai Univ, Fac Pharmaceut Sci, Sakado, Saitama 3500295, Japan
来源：
TOXICOLOGY LETTERS | 1998年 / 99卷 / 03期
关键词：
pefluorocarboxylic acids; gas-liquid chromatography; biological samples;
D O I：
10.1016/S0378-4274(98)00154-4
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
A method for the determination of tissue perfluorocarboxylic acids (PFCA) was developed using gas-liquid chromatography with an electron capture detector (ECD). Perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA) were efficiently extracted from rat liver, methylated with diazomethane, and separated on GLC. Internal standards that were added to liver homogenates were used in the quantitative analysis of PFCAs to correct the loss during the extraction and derivatization. The concentration of PFDA in rat liver 24 h after intraperitoneal administration at a dose of 20 mg/kg body weight was 113.9 +/- 11.4 mu g/g liver. The value corresponds to the previously reported results that were obtained using [C-14]PFDA. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
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页码：183 / 190
页数：8
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