Insight into Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile

被引：210

作者：

Amlacher, Stefan ^{[2
]}

Sarges, Phillip ^{[2
]}

Flemming, Dirk ^{[2
]}

van Noort, Vera ^{[1
]}

Kunze, Ruth ^{[2
]}

Devos, Damien P. ^{[1
]}

Arumugam, Manimozhiyan ^{[1
]}

Bork, Peer ^{[1
]}

Hurt, Ed ^{[2
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] Heidelberg Univ, Zentrum Biochem, D-69120 Heidelberg, Germany

来源：

CELL | 2011年 / 146卷 / 02期

关键词：

OPEN READING FRAMES; MOLECULAR ARCHITECTURE; ELECTRON-MICROSCOPY; BASE COMPOSITION; NEW-GENERATION; YEAST; NUCLEOPORINS; MEMBRANE; EXPORT; TEMPERATURE;

D O I：

10.1016/j.cell.2011.06.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite decades of research, the structure and assembly of the nuclear pore complex (NPC), which is composed of similar to 30 nucleoporins (Nups), remain elusive. Here, we report the genome of the thermophilic fungus Chaetomium thermophilum (ct) and identify the complete repertoire of Nups therein. The thermophilic proteins show improved properties for structural and biochemical studies compared to their mesophilic counterparts, and purified ctNups enabled the reconstitution of the inner pore ring module that spans the width of the NPC from the anchoring membrane to the central transport channel. This module is composed of two large Nups, Nup192 and Nup170, which are flexibly bridged by short linear motifs made up of linker Nups, Nic96 and Nup53. This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines.

引用

页码：277 / 289

页数：13

共 71 条

[1] A simple method for extraction of fungal genomic DNA [J].

Al-Samarrai, TH ;

Schmid, J .

LETTERS IN APPLIED MICROBIOLOGY, 2000, 30 (01) :53-56

[2] The molecular architecture of the nuclear pore complex [J].

Alber, Frank ;

Dokudovskaya, Svetlana ;

Veenhoff, Liesbeth M. ;

Zhang, Wenzhu ;

Kipper, Julia ;

Devos, Damien ;

Suprapto, Adisetyantari ;

Karni-Schmidt, Orit ;

Williams, Rosemary ;

Chait, Brian T. ;

Sali, Andrej ;

Rout, Michael P. .

NATURE, 2007, 450 (7170) :695-701

[3] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[4] Identification of a 60S preribosomal particle that is closely linked to nuclear export [J].

Bassler, J ;

Grandi, P ;

Gadal, O ;

Lessmann, T ;

Petfalski, E ;

Tollervey, D ;

Lechner, J ;

Hurt, E .

MOLECULAR CELL, 2001, 8 (03) :517-529

[5] Functional characterization of a Nup159p-containing nuclear pore subcomplex [J].

Belgareh, N ;

Snay-Hodge, C ;

Pasteau, F ;

Dagher, S ;

Cole, CN ;

Doye, V .

MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (12) :3475-3492

[6] Parallel adaptations to high temperatures in the Archaean eon [J].

Boussau, Bastien ;

Blanquart, Samuel ;

Necsulea, Anamaria ;

Lartillot, Nicolas ;

Gouy, Manolo .

NATURE, 2008, 456 (7224) :942-U74

[7] Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats [J].

Brohawn, Stephen G. ;

Leksa, Nina C. ;

Spear, Eric D. ;

Rajashankar, Kanagalaghatta R. ;

Schwartz, Thomas U. .

SCIENCE, 2008, 322 (5906) :1369-1373

[8] Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice [J].

Brohawn, Stephen G. ;

Schwartz, Thomas U. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2009, 16 (11) :1173-U8

[9] Structures of the tRNA export factor in the nuclear and cytosolic states [J].

Cook, Atlanta G. ;

Fukuhara, Noemi ;

Jinek, Martin ;

Conti, Elena .

NATURE, 2009, 461 (7260) :60-U60

[10] Proteomic analysis of the mammalian nuclear pore complex [J].

Cronshaw, JA ;

Krutchinsky, AN ;

Zhang, WZ ;

Chait, BT ;

Matunis, MJ .

JOURNAL OF CELL BIOLOGY, 2002, 158 (05) :915-927

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