Removal of 5′- and 3′-untranslated regions enhances erythropoietin gene expression in mammalian cells

被引：0
|
作者：
Park, JH [1 ]
Kim, C
Kim, WB
Kim, YK
Lee, SY
Yang, JM
机构：
[1] DonaA Pharm Co Ltd, Res Labs, Yonginsi 449900, Kyunggido, South Korea
[2] Sogang Univ, Dept Life Sci, Seoul 121742, South Korea
[3] Korea Res Inst Biosci & Biotechnol, Taejon 305600, South Korea
[4] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea
来源：
MOLECULAR BIOLOGY | 2001年 / 35卷 / 03期
关键词：
erythropoietin; SR alpha promoter; untranslated region; transcription regulation;
D O I：
10.1023/A:1010466526838
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of the 5 '- and 3 ' -untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5 ' -UTR-deleted, 3 ' -UTR-deleted, and 5 ' ,3 ' -UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5 ' ,3 ' -UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5 ' ,3 ' -UTR-deleted gEpo might he related with the transcription efficiency.
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收藏
页码：350 / 353
页数：4
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