Interleukin-4 induces a switch of human intestinal mast cells from proinflammatory cells to Th2-type cells

被引:26
作者
Bischoff, SC [1 ]
Sellge, G [1 ]
Manns, MP [1 ]
Lorentz, A [1 ]
机构
[1] Hannover Med Sch, Dept Gastroenterol & Hepatol, D-30623 Hannover, Germany
关键词
mast cells; intestinal; bacteria; cytokines;
D O I
10.1159/000053695
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: During the last years, mast cells have been recognized as a potent cellular source of multiple cytokines. However, little is known about the regulation of cytokine production by mature human mast cells derived from mucosal sites. Methods: Human mast cells were isolated from intestinal mucosa and cultured for 14 days in the presence of stem cell factor (SCF) alone or in combination with IL-4. Mast cells were then stimulated by IgE receptor cross-linking or bacterial infection and cytokine production was examined by RT-PCR and ELISA. Results: We found that hu man intestinal mast cells produce proinflammatory cytokines such as TNF-alpha, IL-1 beta and IL-6 without further stimulation. Stimulation of the cells with gram-negative bacteria (Escherichia coli and others) caused an upregulation of TNF-a expression. Following IgE receptor cross-linking, we found additional expression of the Th2 cytokines IL-3, IL-5 and IL-13. Interestingly, mRNA for IL-3, IL-5 and IL-13 was also expressed in unstimulated mast cells provided they were cultured in the presence of SCF and IL-4. Moreover, IL-4 rendered mast cells capable of releasing IL-5 in response to bacterial challenge. Conclusion: In the presence of the mast cell survival factor SCF, mature human mast cells produce predominantly proinflammatory cytokines, whereas in the presence of SCF and IL-4, mast cells produce not only proinflammatory but also Th2 cytokines. Copyright (C) 2001 S. Karger AG, Basel.
引用
收藏
页码:151 / 154
页数:4
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