Toxicity of Bioactive Molecule Andrographolide against Spodoptera litura Fab and Its Binding Potential with Detoxifying Enzyme Cytochrome P450

被引：14

作者：

Edwin, Edward-Sam ^{[1
,2
]}

Vasantha-Srinivasan, Prabhakaran ^{[3
]}

Senthil-Nathan, Sengottayan ^{[1
]}

Chellappandian, Muthiah ^{[4
]}

Karthi, Sengodan ^{[1
]}

Narayanaswamy, Radhakrishnan ^{[5
]}

Stanley-Raja, Vethamonickam ^{[1
]}

Sivanesh, Haridoss ^{[1
]}

Ramasubramanian, Ramakrishnan ^{[1
]}

Al-Huqail, Asma A. ^{[6
]}

Khan, Faheema ^{[6
]}

Krutmuang, Patcharin ^{[7
,8
]}

Abdel-Megeed, Ahmed ^{[9
]}

Ghaith, Aml ^{[10
]}

Paik, Chae-Hoon ^{[11
]}

机构：

[1] Manonmaniam Sundaranar Univ, Div Biopesticides & Environm Toxicol, ASPKCES, Tirunelveli 627412, Tamil Nadu, India

[2] St Peters Med Coll Hosp & Res Inst, Dept Microbiol, Div Virol & Mol Biol, Hosur 635109, Tamil Nadu, India

[3] St Peters Inst Higher Educ & Res, Dept Biotechnol, Chennai 600054, Tamil Nadu, India

[4] VO Chidambaram Coll, Dept Bot, Thoothukudi 628008, Tamil Nadu, India

[5] St Peters Inst Higher Educ & Res, Dept Biochem, Sch Life Sci, Chennai 600054, Tamil Nadu, India

[6] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia

[7] Chiang Mai Univ, Fac Agr, Dept Entomol & Plant Pathol, Chiang Mai 50200, Thailand

[8] Chiang Mai Univ, Fac Agr, Innovat Agr Res Ctr, Chiang Mai 50200, Thailand

[9] Alexandria Univ, Fac Agr Saba Basha, Dept Plant Protect, Alexandria 21531, Egypt

[10] Derna Univ, Fac Sci, Dept Zool, Derna 417230, Libya

[11] Natl Inst Crop Sci, Planning & Coordinat Div, RDA, 181 Hyeoksin Ro, Jeollabuk Do 55365, South Korea

来源：

MOLECULES | 2021年 / 26卷 / 19期

关键词：

andrographolide; RGR; RCR; ECI; ECD; molecular docking; PLUTELLA-XYLOSTELLA LEPIDOPTERA; MEDINALIS GUENEE LEPIDOPTERA; NUTRITIONAL PHYSIOLOGY; HELICOVERPA-ARMIGERA; CRUDE EXTRACTS; DIAMONDBACK MOTH; AMYLASE ACTIVITY; MELIA-AZEDARACH; TROPICAL PLANT; PANICULATA;

D O I：

10.3390/molecules26195982

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spodoptera litura Fab. is a polyphagous pest causing damage to many agriculture crops leading to yield loss. Recurrent usage of synthetic pesticides to control this pest has resulted in resistance development. Plant-derived diterpenoid compound andrographolide was isolated from the leaves of Andrographis paniculata. It was analysed by gas chromatography-mass spectroscopy and quantified by HPLC. Nutritional indices and digestive enzymatic profile were evaluated. Third, fourth and fifth instar larvae were treated with different concentrations of andrographolide. At 3, 6 and 9 ppm-treated concentrations the larvae showed decreased RGR, RCR, ECI, ECD values with adverse increase in AD. The digestive enzymes were significantly inhibited when compared with control. Conspicuously, andrographolide showed pronounced mortality of S. litura by inhibition of enzyme secretion and intake of food. The binding ability of andrographolide with CYTP450 showed high affinity with low binding energy. Andrographolide has the potential to be exploited as a biocontrol agent against S. litura as an eco-friendly pesticide.</p>

引用

页数：13

共 60 条

[1] A method of computing the effectiveness of an insecticide [J].