Tumor necrosis factor-α, interleukin-1β and interleukin-6 in the cerebrospinal fluid of newborn with meningitis

被引:23
作者
Krebs, VLJ
Okay, TS
Okay, Y
Vaz, FAC
机构
[1] Hosp Clin FMUSP, Ins Crianca, Unidade Cuidados Intens Neonatal, Livre Docente Pediat,Med Assistente Encarregada S, Sao Paulo, Brazil
[2] FMUSP, Coutora Ciencias, Lab Invest Med 36, Sao Paulo, Brazil
[3] FMUSP, Dept Pediat, Sao Paulo, Brazil
[4] Univ Sao Paulo, Sch Med, Dept Pediat, FMUSP, Sao Paulo, Brazil
关键词
meningitis; cerebrospinal fluid; newborn; tumor necrosis factor-alpha; interleukin-1; beta; interleukin-6;
D O I
10.1590/S0004-282X2005000100002
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Objective: To analyze the usefulness of determining the cerebrospinal fluid(CSF) levels of tumor necrosis factor-alpha(TNF-alpha),interleukin-1 beta(IL-1 beta) and interieukin-6(IL-6)for the early diagnosis and evaluation of the prognosis of neonatal meningitis. Method: We studied 54 newborn that underwent lumbar puncture. Thirty patients had meningitis and 24 were the control group. CSF and sera were obtained at the moment of suspicion of meningitis and stored at -70 degrees C. Cytokines were performed by enzyme-linked immunosorbent assay method. Results: CSF cytokines were detected in all the newborn with meningitis. TNF-alpha was detected in the CSF in 63.3% of the neonates, IL-1 beta in 73.3% and IL-6 in 96.6%. The CSF levels were significantly higher than serum in neonates with meningitis. There was no correlation between the CSF levels of cytokines and neurologic complications. Conclusion: The detection of TNF-alpha, IL-1 beta and IL-6 in the CSF is of great value in order to achieve a early diagnosis of neonatal meningitis. Among the three cytokines analyzed, IL-6 was the best indicator of meningeal inflammation.
引用
收藏
页码:7 / 13
页数:7
相关论文
共 21 条
  • [11] INCREASED ENDOTOXIN AND INTERLEUKIN-1-BETA CONCENTRATIONS IN CEREBROSPINAL-FLUID OF INFANTS WITH COLIFORM MENINGITIS AND VENTRICULITIS ASSOCIATED WITH INTRAVENTRICULAR GENTAMICIN THERAPY
    MUSTAFA, MM
    MERTSOLA, J
    RAMILO, O
    SAEZLLORENS, X
    RISSER, RC
    MCCRACKEN, GH
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1989, 160 (05) : 891 - 895
  • [12] CEREBROSPINAL-FLUID PROSTAGLANDINS, INTERLEUKIN-1-BETA, AND TUMOR-NECROSIS-FACTOR IN BACTERIAL-MENINGITIS - CLINICAL AND LABORATORY CORRELATIONS IN PLACEBO-TREATED AND DEXAMETHASONE-TREATED PATIENTS
    MUSTAFA, MM
    RAMILO, O
    SAEZLLORENS, X
    OLSEN, KD
    MAGNESS, RR
    MCCRACKEN, GH
    [J]. AMERICAN JOURNAL OF DISEASES OF CHILDREN, 1990, 144 (08): : 883 - 887
  • [13] Neonatal meningitis: Mortality, cerebrospinal fluid, and microbiological findings
    Nel, E
    [J]. JOURNAL OF TROPICAL PEDIATRICS, 2000, 46 (04) : 237 - 239
  • [14] CEREBROSPINAL-FLUID CONCENTRATIONS OF INTERLEUKIN-1-BETA, TUMOR-NECROSIS-FACTOR-ALPHA, AND INTERFERON-GAMMA IN BACTERIAL-MENINGITIS
    OHGA, S
    AOKI, T
    OKADA, K
    AKEDA, H
    FUJIOKA, K
    OHSHIMA, A
    MORI, T
    MINAMISHIMA, I
    UEDA, K
    [J]. ARCHIVES OF DISEASE IN CHILDHOOD, 1994, 70 (02) : 123 - 125
  • [15] OSAVMAN K, 1998, PEDIAT RS, V43, P476
  • [17] The efficacy of pentoxifylline as an anti-inflammatory agent in experimental Escherichia coli meningitis in the newborn piglet
    Park, WS
    Chang, YS
    Lee, M
    [J]. BIOLOGY OF THE NEONATE, 2000, 77 (04): : 236 - 242
  • [18] TUMOR NECROSIS FACTOR-ALPHA CACHECTIN AND INTERLEUKIN-1-BETA INITIATE MENINGEAL INFLAMMATION
    RAMILO, O
    SAEZLLORENS, X
    MERTSOLA, J
    JAFARI, H
    OLSEN, KD
    HANSEN, EJ
    YOSHINAGA, M
    OHKAWARA, S
    NARIUCHI, H
    MCCRACKEN, GH
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (02) : 497 - 507
  • [19] INTERLEUKIN-6 ACTIVITY IN INFANTS AND CHILDREN WITH BACTERIAL-MENINGITIS
    RUSCONI, F
    PARIZZI, F
    GARLASCHI, L
    ASSAEL, BM
    SIRONI, M
    GHEZZI, P
    MANTOVANI, A
    [J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1991, 10 (02) : 117 - 121
  • [20] MENINGEAL INFLAMMATION IN NEONATAL GRAM-NEGATIVE BACTEREMIA
    SARMAN, G
    MOISE, AA
    EDWARDS, MS
    [J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1995, 14 (08) : 701 - 704