Self-assembled adhesive biomaterials formed by a genetically designed fusion protein

被引：17

作者：

Aich, Pulakesh ^{[1
]}

An, Jaeyeon ^{[2
]}

Yang, Byeongseon ^{[3
]}

Ko, Young Ho ^{[1
]}

Kim, Junghyun ^{[1
]}

Murray, James ^{[1
]}

Cha, Hyung Joon ^{[3
]}

Roh, Joon Ho ^{[1
]}

Park, Kyeng Min ^{[1
]}

Kim, Kimoon ^{[1
,2
]}

机构：

[1] Inst for Basic Sci Korea, Ctr Self Assembly & Complex, Pohang 37673, South Korea

[2] Pohang Univ Sci & Technol, Dept Chem, Pohang 37673, South Korea

[3] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 89期

关键词：

SPIDER SILK; UNDERWATER ADHESIVES; MUSSEL; MECHANISMS; EXPRESSION; HYDROGELS; TOUGH;

D O I：

10.1039/c8cc07475e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here we report a recombinant protein (MS) obtained by genetic fusion of a mussel foot protein (Mfp3) motif into a silk spidroin (MaSp1). The MS not only self-assembled into a supramolecular fibre, as does the parent MaSp1, but also showed enhanced adhesiveness resulting from the DOPA-containing Mfp3 portion. The successful incorporation of the wet adhesiveness of Mfp3 into the well-structured assembly of MaSp1 may provide a new insight for the genetic design of underwater adhesive recombinant proteins by utilizing the structural features of a spidroin protein.

引用

页码：12642 / 12645

页数：4

共 31 条

[1]

Ahn BK, 2014, NAT MATER, V13, P867, DOI [10.1038/nmat4037, 10.1038/NMAT4037]

[2] Biomechanical properties of Achilles tendon repair augmented with a bioadhesive-coated scaffold [J].

Brodie, Michael ;

Vollenweider, Laura ;

Murphy, John L. ;

Xu, Fangmin ;

Lyman, Arinne ;

Lew, William D. ;

Lee, Bruce P. .

BIOMEDICAL MATERIALS, 2011, 6 (01)

[3] Nonlinear material behaviour of spider silk yields robust webs [J].

Cranford, Steven W. ;

Tarakanova, Anna ;

Pugno, Nicola M. ;

Buehler, Markus J. .

NATURE, 2012, 482 (7383) :72-U91

[4] Highly Extensible, Tough, and Elastomeric Nanocomposite Hydrogels from Poly(ethylene glycol) and Hydroxyapatite Nanoparticles [J].

Gaharwar, Akhilesh K. ;

Dammu, Sandhya A. ;

Canter, Jamie M. ;

Wu, Chia-Jung ;

Schmidt, Gudrun .

BIOMACROMOLECULES, 2011, 12 (05) :1641-1650

[5] Mussel-Inspired Adhesive and Tough Hydrogel Based on Nanoclay Confined Dopamine Polymerization [J].

Han, Lu ;

Lu, Xiong ;

Liu, Kezhi ;

Wang, Kefeng ;

Fang, Liming ;

Weng, Lu-Tao ;

Zhang, Hongping ;

Tang, Youhong ;

Ren, Fuzeng ;

Zhao, Cancan ;

Sun, Guoxing ;

Liang, Rui ;

Li, Zongjin .

ACS NANO, 2017, 11 (03) :2561-2574

[6] METHODS AND REAGENTS - PURIFICATION OF HIS-TAG FUSION PROTEINS FROM ESCHERICHIA-COLI [J].

HENGEN, PN .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (07) :285-286

[7] Bioinspired Underwater Adhesives by Using the Supramolecular Toolbox [J].

Hofman, Anton H. ;

van Hees, Ilse A. ;

Yang, Juan ;

Kamperman, Marleen .

ADVANCED MATERIALS, 2018, 30 (19)

[8] Polymerizing Like Mussels Do: Toward Synthetic Mussel Foot Proteins and Resistant Glues [J].

Horsch, Justus ;

Wilke, Patrick ;

Pretzler, Matthias ;

Seuss, Maximilian ;

Melnyk, Inga ;

Remmler, Dario ;

Fery, Andreas ;

Rompel, Annette ;

Boerner, Hans G. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (48) :15728-15732

[9] Molecular mechanisms of spider silk [J].

Hu, X. ;

Vasanthavada, K. ;

Kohler, K. ;

McNary, S. ;

Moore, A. M. F. ;

Vierra, C. A. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2006, 63 (17) :1986-1999

[10] Pulsating Tubules from Noncovalent Macrocycles [J].

Huang, Zhegang ;

Kang, Seong-Kyun ;

Banno, Motonori ;

Yamaguchi, Tomoko ;

Lee, Dongseon ;

Seok, Chaok ;

Yashima, Eiji ;

Lee, Myongsoo .

SCIENCE, 2012, 337 (6101) :1521-1526

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