Effect of Light on Iron Uptake by the Freshwater Cyanobacterium Microcystis aeruginosa

被引：58
作者：
Fujii, M. [1 ]
Dang, T. C. [1 ]
Rose, A. L. [1 ,2 ]
Omura, T. [3 ]
Waite, T. D. [1 ]
机构：
[1] Univ New S Wales, Sch Civil & Environm Engn, Sydney, NSW 2052, Australia
[2] So Cross Univ, Lismore, NSW 2480, Australia
[3] Tohoku Univ, Grad Sch Engn, Dept Civil & Environm Engn, Sendai, Miyagi 9808579, Japan
来源：
ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2011年 / 45卷 / 04期
基金：
日本学术振兴会; 澳大利亚研究理事会;
关键词：
SURFACE OCEAN; PHYTOPLANKTON; ACQUISITION; FE; SPECIATION; KINETICS; BIOAVAILABILITY; SUPEROXIDE; SEAWATER; FE(II);
D O I：
10.1021/es103311h
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Visible light was observed to induce reductive dissociation of organically complexed Fe and dramatically increase the short-term uptake rate of radiolabeled Fe by Microcystis aeruginosa PCC7806 in Fraquil* medium buffered by a single metal chelator, ethylenediaminetetraacetic acid (EDTA). Only wavelengths <500 nm activated Fe uptake indicating that Fe photochemistry rather than biological factors is responsible for the facilitated uptake. The measured rate of photochemical Fe(II) production combined with a significant decrease in Fe-55 uptake rate in the presence of ferrozine (a strong ferrous iron chelator) confirmed that photogenerated unchelated Fe(II) was the major form of Fe taken up by M. aeruginosa under the conditions examined. Mathematical modeling based on unchelated Fe(II) uptake by concentration gradient dependent passive diffusion of Fe(II) through nonspecific transmembrane channels (porins) could account for the magnitude of Fe uptake and a variety of other observations such as the effect of competing ligands on Fe uptake. Steady-state uptake rates indicated that M. aeruginosa acquires Fe predominantly during the light cycle. This study confirms that Fe photochemistry has a dominant impact on Fe acquisition and growth by M. aeruginosa in EDTA-buffered culture medium.
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收藏
页码：1391 / 1398
页数：8
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