Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae

被引:358
|
作者
Davidson, JF [1 ]
Whyte, B [1 ]
Bissinger, PH [1 ]
Schiestl, RH [1 ]
机构
[1] HARVARD UNIV,SCH PUBL HLTH,DEPT MOLEC & CELLULAR TOXICOL,BOSTON,MA 02115
关键词
D O I
10.1073/pnas.93.10.5116
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The cause for death after lethal heat shock is not well understood. A shift from low to intermediate temperature causes the induction of heat-shock proteins in most organisms. However, except for HSP104, a convincing involvement of heat-shock proteins in the development of stress resistance has not been established in Saccharomyces cerevisiae. This paper shows that oxidative stress and antioxidant enzymes play a major role in heat-induced cell death in yeast. Mutants deleted for the antioxidant genes catalase, superoxide dismutase, and cytochrome c peroxidase were more sensitive to the lethal effect of heat than isogenic wild-type cells. Overexpression of catalase and superoxide dismutase genes caused an increase in thermotolerance. Anaerobic conditions caused a 500- to 20,000-fold increase in thermotolerance. The thermotolerance of cells in anaerobic conditions was immediately abolished upon oxygen exposure. HSP104 is not responsible for the increased resistance of anaerobically grown cells. The thermotolerance of anaerobically grown cells is not due to expression of heat-shock proteins. By using an oxidation-dependent fluorescent molecular probe a 2- to 3-fold increase in fluorescence was found upon heating. Thus, we conclude that oxidative stress is involved in heat-induced cell death.
引用
收藏
页码:5116 / 5121
页数:6
相关论文
共 50 条
  • [1] Polyethylene terephthalate nanoplastics cause oxidative stress induced cell death in Saccharomyces cerevisiae
    Kaluc, Nur
    Cotelli, E. Lal
    Tuncay, Salih
    Thomas, Pinar B.
    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, 2024, 59 (04): : 180 - 188
  • [2] Mitochondrial respiratory electron carriers are involved in oxidative stress during heat stress in Saccharomyces cerevisiae
    Davidson, JF
    Schiestl, RH
    MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (24) : 8483 - 8489
  • [3] The Response to Heat Shock and Oxidative Stress in Saccharomyces cerevisiae
    Morano, Kevin A.
    Grant, Chris M.
    Moye-Rowley, W. Scott
    GENETICS, 2012, 190 (04) : 1157 - 1195
  • [4] Tyrosine is involved in protection from oxidative stress in Saccharomyces cerevisiae
    Lupo, S
    Aranda, C
    MirandaHam, L
    Olivera, H
    Riego, L
    Servin, L
    Gonzalez, A
    CANADIAN JOURNAL OF MICROBIOLOGY, 1997, 43 (10) : 963 - 970
  • [5] Zea mays leaf extracts protect Saccharomyces cerevisiae cell against oxidative stress-induced cell death
    Kiruthika, Balasubramanian
    Padma, Palghat Raghunathan
    JOURNAL OF ACUTE MEDICINE, 2013, 3 (03) : 83 - 92
  • [6] Role of glutathione in heat-shock-induced cell death of Saccharomyces cerevisiae
    Sugiyama, K
    Kawamura, A
    Izawa, S
    Inoue, Y
    BIOCHEMICAL JOURNAL, 2000, 352 : 71 - 78
  • [7] HEAT-INDUCED ACCUMULATION AND FUTILE CYCLING OF TREHALOSE IN SACCHAROMYCES-CEREVISIAE
    HOTTIGER, T
    SCHMUTZ, P
    WIEMKEN, A
    JOURNAL OF BACTERIOLOGY, 1987, 169 (12) : 5518 - 5522
  • [8] Depression by miconazole of heat-induced respiratory-deficiency in Saccharomyces cerevisiae
    Madeira-Lopes, A
    Miranda, J
    JOURNAL OF BASIC MICROBIOLOGY, 1998, 38 (01) : 27 - 31
  • [9] Cadmium-induced oxidative stress in Saccharomyces cerevisiae
    Muthukumar, Kannan
    Nachiappan, Vasanthi
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2010, 47 (06): : 383 - 387
  • [10] Role of Oxidative Phosphorylation in Histatin 5-Induced Cell Death in Saccharomyces cerevisiae
    Kris De Smet
    Rieka Reekmans
    Roland Contreras
    Biotechnology Letters, 2004, 26 : 1781 - 1785