Sperm Quality in Young Bull Semen Can Be Improved by Single Layer Centrifugation

被引:3
|
作者
Lima-Verde, Isabel [1 ]
Hurri, Emma [2 ]
Ntallaris, Theodoros [1 ]
Johannisson, Anders [1 ]
Stalhammar, Hans [2 ]
Morrell, Jane M. [1 ]
机构
[1] Swedish Univ Agr Sci, Clin Sci, Almas Alle 8, S-75007 Uppsala, Sweden
[2] VikingGenetics, S-53294 Skara, Sweden
来源
ANIMALS | 2022年 / 12卷 / 18期
关键词
sperm quality; young bull ejaculates; genomic selection; genetic progress; generation interval; single-layer centrifugation; sperm cryopreservation; ANDROCOLL-E; SPERMATOZOA; FERTILITY; SELECTION;
D O I
10.3390/ani12182435
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Simple Summary Genomic selection enables bulls with desirable genes to be identified early in life. Livestock producers need to use the semen from young bulls as early as possible for efficient milk and meat production with fewer greenhouse gas emissions. However, semen from young bulls is often of lower quality than needed for freezing for commercial artificial insemination. Colloid centrifugation selects spermatozoa with the desirable characteristics needed for fertilization from the rest of the ejaculate. In this study, split ejaculates from young bulls were prepared with or without colloid centrifugation. Using this technique, sperm doses of acceptable quality for artificial insemination could be produced from ejaculates that would otherwise be discarded. Thus, the semen from young bulls would be usable for artificial insemination sooner than is currently the case. Interest in using semen from young bulls is increasing due to identifying promising animals by genomic selection. However, sperm quality in these ejaculates may not reach currently accepted standards for the cattle breeding industry. The purpose of this study was to determine if centrifugation of semen from young bulls through the Bovicoll colloid could improve sperm quality sufficiently for the frozen semen to be acceptable for artificial insemination. Ejaculates from 19 young bulls were split and either processed by Single-Layer Centrifugation (SLC) or not (CON) before freezing. After thawing, sperm quality was evaluated by determination of membrane integrity, mitochondrial membrane potential, DNA integrity, production of reactive oxygen species, sperm morphology and motility. Approximately half of the CON samples reached acceptable post-thaw quality (membrane integrity >= 40%) despite being below the breeding company ' s desired sperm concentration threshold pre-freezing. In the remaining samples, sperm quality was improved by SLC such that 45% of them reached acceptable quality post-thaw. Almost 75% of the young bull sperm samples could have produced usable frozen semen doses by adjusting the breeding company ' s current processing protocols. Since lowering the generation interval has a direct effect on the genetic gain per year, SLC could aid genetic progress in cattle breeding.
引用
收藏
页数:14
相关论文
共 50 条
  • [31] Practical guide to single layer centrifugation of stallion semen
    Morrell, J. M.
    Nunes, M. M.
    EQUINE VETERINARY EDUCATION, 2018, 30 (07) : 392 - 398
  • [32] Single layer centrifugation with Androcoll-E™ improves the quality of cooled stallion semen
    Costa, A. L.
    Martins-Bessa, A.
    Rebello de Andrade, A.
    Guimaraes, T.
    Rebordao, M. R.
    Gamboa, S.
    Bravo, P. P.
    Correia, M. J.
    Gaivao, I.
    Rocha, A.
    REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 : 116 - 116
  • [33] Bull sperm surface "craters" and other aspects of semen quality
    Foote, RH
    THERIOGENOLOGY, 1999, 51 (04) : 767 - 775
  • [34] Use of colloid single layer centrifugation for dromedary camel semen: Effect of initial dilution and comparison of two colloids on sperm quality parameters
    Malo, C.
    Morrell, J. M.
    Crichton, E. G.
    Pukazhenthi, B. S.
    Skidmore, J. A.
    ANIMAL REPRODUCTION SCIENCE, 2016, 169 : 123 - 123
  • [35] Effect of rosmarinic acid on bull sperm quality and bacteria in semen
    Hagos, Lidya
    Cojkic, Aleksandar
    Hanson, Ingrid
    Johannisson, Anders
    Ntallaris, Theodoros
    Morrell, Jane M.
    ANIMAL REPRODUCTION SCIENCE, 2020, 220
  • [36] Effect of single-layer density centrifugation on the quality of cryopreserved sperm of Cuniculus paca
    Daniela Munera, C.
    Giovanni Restrepo, B.
    REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU, 2023, 34 (05):
  • [37] Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses
    Ortiz, I.
    Dorado, J.
    Ramirez, L.
    Morrell, J. M.
    Acha, D.
    Urbano, M.
    Galvez, M. J.
    Carrasco, J. J.
    Gomez-Arrones, V.
    Calero-Carretero, R.
    Hidalgo, M.
    ANIMAL, 2014, 8 (02) : 308 - 315
  • [38] Effect of Single Layer Centrifugation on reactive oxygen species and sperm mitochondrial membrane potential in cooled stallion semen
    Morrell, J. M.
    Lagerqvist, A.
    Humblot, P.
    Johannisson, A.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2017, 29 (05) : 1039 - 1045
  • [39] Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates
    Gloria, Alessia
    Carluccio, Augusto
    Wegher, Laura
    Robbe, Domenico
    Befacchia, Giovanni
    Contri, Alberto
    JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, 2016, 7
  • [40] Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates
    Alessia Gloria
    Augusto Carluccio
    Laura Wegher
    Domenico Robbe
    Giovanni Befacchia
    Alberto Contri
    Journal of Animal Science and Biotechnology, 7