A Structurally Distinct Human Mycoplasma Protein that Generically Blocks Antigen-Antibody Union

被引：73

作者：

Grover, Rajesh K. ^{[1
,2
]}

Zhu, Xueyong ^{[3
]}

Nieusma, Travis ^{[3
]}

Jones, Teresa ^{[1
]}

Boero, Isabel ^{[1
]}

MacLeod, Amanda S. ^{[4
]}

Mark, Adam ^{[5
]}

Niessen, Sherry ^{[6
]}

Kim, Helen J. ^{[3
]}

Kong, Leopold ^{[3
]}

Assad-Garcia, Nacyra ^{[7
]}

Kwon, Keehwan ^{[7
]}

Chesi, Marta ^{[8
]}

Smider, Vaughn V. ^{[1
]}

Salomon, Daniel R. ^{[5
]}

Jelinek, Diane F. ^{[9
,10
]}

Kyle, Robert A. ^{[9
,10
]}

Pyles, Richard B. ^{[11
,12
]}

Glass, John I. ^{[7
]}

Ward, Andrew B. ^{[3
]}

Wilson, Ian A. ^{[3
,13
]}

Lerner, Richard A. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Cell & Mol Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA

[5] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

[6] Scripps Res Inst, Ctr Physiol Prote, La Jolla, CA 92037 USA

[7] J Craig Venter Res Inst, Synthet Biol & Bioenergy Grp, Rockville, MD 20850 USA

[8] Mayo Clin Arizona, Comprehens Canc Ctr, Scottsdale, AZ 85259 USA

[9] Mayo Clin, Div Hematol, Dept Internal Med, Coll Med, Rochester, MN 55905 USA

[10] Mayo Clin, Coll Med, Dept Immunol, Rochester, MN 55905 USA

[11] Univ Texas Med Branch, Dept Pediat, Galveston, TX 77555 USA

[12] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA

[13] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

SCIENCE | 2014年 / 343卷 / 6171期

关键词：

MULTIPLE-MYELOMA; BINDING DOMAIN; EBOLA-VIRUS; FAB; RECOGNITION; EPITOPES; COMPLEX;

D O I：

10.1126/science.1246135

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the kappa and lambda light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.

引用

页码：656 / 661

页数：6

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