Chip-Based Free-Flow Electrophoresis with Integrated Nanospray Mass-Spectrometry

被引：55
作者：
Benz, Christian [1 ,3 ]
Boomhoff, Michael [2 ]
Appun, Johannes [2 ]
Schneider, Christoph [2 ]
Belder, Detlev [1 ]
机构：
[1] Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
[2] Univ Leipzig, Inst Organ Chem, D-04103 Leipzig, Germany
[3] Univ Leipzig, LIFE Leipzig Res Ctr Civilizat Dis, D-04109 Leipzig, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 09期
关键词：
cycloaddition; continuous micro flow-through; electrophoresis; mass spectrometry; microfluidics; ON-A-CHIP; ZONE ELECTROPHORESIS; MICROFLUIDICS; MICROREACTORS; ELECTROSPRAY; CHEMISTRY; MICROCHIP; ONLINE; TECHNOLOGY; REACTORS;
D O I：
10.1002/anie.201409663
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Free-flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free-flow electrophoresis and mass spectrometry it is now also possible to trace non-fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]-cycloannulation.
引用
收藏
页码：2766 / 2770
页数：5
相关论文
共 50 条
  • [41] Chip-based capillary electrophoresis platforms: toward point-of-care applications
    Eijkel, Jan
    BIOANALYSIS, 2015, 7 (11) : 1385 - 1387
  • [42] SEPARATION OF AMINO-ACIDS BY RECYCLING FREE-FLOW ELECTROPHORESIS
    ZHENG, SN
    HANAI, T
    YONEMOTO, T
    TADAKI, T
    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1992, 25 (06) : 686 - 691
  • [43] Counterflow Gradient Focusing in Free-Flow Electrophoresis for Protein Fractionation
    Courtney, Matthew
    Thompson, Ethan
    Glawdel, Tomasz
    Ren, Carolyn L.
    ANALYTICAL CHEMISTRY, 2020, 92 (10) : 7317 - 7324
  • [44] On-chip free-flow magnetophoresis: Separation and detection of mixtures of magnetic particles in continuous flow
    Pamme, Nicole
    Eijkel, Jan C. T.
    Manz, Andreas
    JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2006, 307 (02) : 237 - 244
  • [45] Micro free-flow isoelectric focusing with integrated optical pH sensors
    Nagl, Stefan
    ENGINEERING IN LIFE SCIENCES, 2018, 18 (02): : 114 - 123
  • [46] Analysis of recombinant adenoviruses using an integrated microfluidic chip-based system
    McCaman, MT
    Murakami, P
    Pungor, E
    Hahnenberger, KM
    Hancock, WS
    ANALYTICAL BIOCHEMISTRY, 2001, 291 (02) : 262 - 268
  • [47] Target protein separation and preparation by free-flow electrophoresis coupled with charge-to-mass ratio analysis
    Shen, Qiao-Yi
    Guo, Chen-Gang
    Yan, Jian
    Zhang, Qiang
    Xie, Hai-Yang
    Jahan, Sharmin
    Fan, Liu-Yin
    Xiao, Hua
    Cao, Cheng-Xi
    JOURNAL OF CHROMATOGRAPHY A, 2015, 1397 : 73 - 80
  • [48] Neuroproteomics Chip-Based Mass Spectrometry and Other Techniques for Alzheimer's Disease Biomarkers - Update
    Pomilio, Alicia B.
    Vitale, Arturo A.
    Lazarowski, Alberto J.
    CURRENT PHARMACEUTICAL DESIGN, 2022, 28 (17) : 1124 - 1151
  • [49] On-line electrochemical tagging of free cysteines during nanospray ionisation for mass spectrometry analysis
    Roussel, C
    Dayon, L
    Rohner, TC
    Lion, N
    Girault, HH
    BIOPHOTONICS NEW FRONTIER: FROM GENOME TO PROTEOME, 2004, 5461 : 30 - 37
  • [50] Novel chip-based isoelectric focusing device for fractionation of bacteria prior to their mass spectrometry identification
    Dusa, Filip
    Salplachta, Jiri
    Horka, Marie
    Lunerova, Kamila
    Rosenbergova, Katerina
    Kubicek, Oldrich
    ANALYTICA CHIMICA ACTA, 2022, 1192
12345