Chip-Based Free-Flow Electrophoresis with Integrated Nanospray Mass-Spectrometry

被引：55
作者：
Benz, Christian [1 ,3 ]
Boomhoff, Michael [2 ]
Appun, Johannes [2 ]
Schneider, Christoph [2 ]
Belder, Detlev [1 ]
机构：
[1] Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
[2] Univ Leipzig, Inst Organ Chem, D-04103 Leipzig, Germany
[3] Univ Leipzig, LIFE Leipzig Res Ctr Civilizat Dis, D-04109 Leipzig, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 09期
关键词：
cycloaddition; continuous micro flow-through; electrophoresis; mass spectrometry; microfluidics; ON-A-CHIP; ZONE ELECTROPHORESIS; MICROFLUIDICS; MICROREACTORS; ELECTROSPRAY; CHEMISTRY; MICROCHIP; ONLINE; TECHNOLOGY; REACTORS;
D O I：
10.1002/anie.201409663
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Free-flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free-flow electrophoresis and mass spectrometry it is now also possible to trace non-fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]-cycloannulation.
引用
收藏
页码：2766 / 2770
页数：5
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