Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

被引：2

作者：

Ghassemi, Saba ^{[1
]}

Milone, Michael C. ^{[1
]}

机构：

[1] Univ Penn, Perelman Sch Med, Ctr Cellular Immunotherapies, Philadelphia, PA 19104 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 154期

关键词：

Cancer Research; Issue; 154; adoptive immunotherapy; chimeric antigen receptors; CAR T cell manufacturing; cancer; T cell; CAR T cell differentiation; MEMORY; THERAPY; IL-7;

D O I：

10.3791/59949

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adoptive immunotherapy holds promise for the treatment of cancer and infectious disease. We describe a simple approach to transduce primary human T cells with chimeric antigen receptor (CAR) and expand their progeny ex vivo. We include assays to measure CAR expression as well as differentiation, proliferative capacity and cytolytic activity. We describe assays to measure effector cytokine production and inflammatory cytokine secretion in CAR T cells. Our approach provides a reliable and comprehensive method to culture CAR T cells for preclinical models of adoptive immunotherapy.

引用

页数：6

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